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Cell Biology & Epigenetics


Products >  Cell_Biology_and_Epigenetics >  Cancer_and_Inflammation >  Osteopontin

Osteopontin Detection

Osteopontin (OPN) is a calcium-binding glycosylated phosphoprotein secreted by many cell types, including osteoblasts, kidney tubule cells, macrophages, activated T cells, and vascular smooth muscle cells. Osteopontin is post-translationally modified; the molecular weight varies within the range of 44–66 kDa, depending on the level of glycosylation and phosphorylation. Thrombin cleaves osteopontin into N-terminal and C-terminal fragments. A variety of OPN isoforms exist within the cell, produced by differential splicing, post-translational modification, and proteolytic cleavage.

OPN contains an Arg-Gly-Asp (RGD) amino acid motif that is also found in other extracellular proteins that bind members of the integrin family of cell-surface receptors. This binding activity has been localized to the N-terminal fragment of osteopontin, generated by thrombin cleavage.

Osteopontin is also known as Spp-1, Apl-1, BNSP, bone sialoprotein 1, ETA-1, and minopontin.

ELISAs for Osteopontin Detection

These kits are solid-phase sandwich ELISAs using two antibodies that are highly specific to human, mouse, or rat OPN or its N-terminal fragments; one is precoated on the ELISA plate and the other is HRP-conjugated. These assay kits can be used to measure soluble human, mouse, or rat OPN protein in serum, EDTA plasma, synovial fluid, urine, or cell culture supernatants.

Antibodies for Osteopontin Detection

These products are affinity-purified IgG antibodies that recognize human, mouse, or rat OPN, or N-terminal fragments of OPN and OPN (O-17). The antibodies were raised in mouse or rabbit using a synthetic peptide and can be used for Western blot (WB) detection or immunohistochemical (IHC) detection of human OPN.

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Features

Antigen
Cat. #
Application
Species
(Clone) and Source
Cross‑Reactivity
Osteopontin
(N‑terminus)
27158AELISAHumanCapture:
(O-17)
Rabbit IgG

Detect: HRP (10A16)
Mouse IgG MoAb
0.2% cross‑reactivity with mouse OPN

≤0.1% cross-reactivity with rat OPN
Osteopontin27351AELISAMouseCapture:
(O-17)
Rabbit IgG

Detect: HRP (O-165)
Rabbit IgG
14.8% cross-reactivity with rat OPN

4.3% cross-reactivity with human OPN
Osteopontin27360AELISARatCapture:
(O-17)
Rabbit IgG

Detect: HRP (O-165)
Rabbit IgG
≤0.1% cross-reactivity with mouse and human OPN
Osteopontin
N-half
27258AELISAHumanCapture:
(34E3)
Mouse IgG MoAb

Detect: HRP (O-17)
Rabbit IgG
n/a
Osteopontin
N-half
27259AELISAMouseCapture:
(34E3)
Mouse IgG MoAb

Detect: HRP (O-17)
Rabbit IgG
n/a
Osteopontin10011AIHC
WB
Human(10A16)
Mouse IgG1 MoAb
Does not cross-react with mouse/rat OPN
Osteopontin
(C‑terminus)
10191A, BIHC
WB
Human(1B20)
Mouse IgG MoAb
Cross-reacts with rabbit OPN
Osteopontin
(N‑terminus)
18621A, BIHC
WB
Mouse(O-17)
Rabbit IgG
Cross-reacts with rat OPN

Does not cross-react with human OPN
Osteopontin
(N‑terminus)
18625A, BIHC
WB
Human(O-17)
Rabbit IgG
Does not cross-react with mouse OPN

Cross-reacts with the thrombin-digested form of human OPN
Osteopontin
(N‑terminus)
18628A, BIHC
WB
Rat(O-17)
Rabbit IgG
Does not cross-react with mouse or human OPN

Cross-reacts with the thrombin-digested form of rat OPN
Osteopontin
(N‑half)
11108A, BWBHuman(34E3)
Mouse IgG MoAb
Cross-reacts with rat and mouse OPN N-half

Does not cross-react with full-length OPN

Additional Information

Please see a product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price Units Select
27158A Human Osteopontin Assay Kit - IBL 96-Well $606.00
27351A Mouse Osteopontin Assay Kit - IBL 96-Well $606.00
10011A Anti-Human Osteopontin (10A16) Mouse IgG MoAb 200 ug $499.00
10191A Anti-Human Osteopontin (1B20) Mouse IgG MoAb 10 ug $113.00
10191B Anti-Human Osteopontin (1B20) Mouse IgG MoAb 100 ug $499.00
11108A Anti-Osteopontin N-Half (34E3) Mouse IgG MoAb 10 ug $113.00
11108B Anti-Osteopontin N-Half (34E3) Mouse IgG MoAb 100 ug $499.00
18621A Anti-Mouse Osteopontin (O-17) Rabbit IgG Affinity Purify 10 ug $141.00
18621B Anti-Mouse Osteopontin (O-17) Rabbit IgG Affinity Purify 100 ug $499.00
18625A Anti-Human Osteopontin (O-17) Rabbit IgG Affinity Purify 10 ug $113.00
18625B Anti-Human Osteopontin (O-17) Rabbit IgG Affinity Purify 100 ug $499.00
18628A Anti-Rat Osteopontin (O-17) Rabbit IgG Affinity Purify 10 ug $113.00
18628B Anti-Rat Osteopontin (O-17) Rabbit IgG Affinity Purify 100 ug $499.00
27258A Human Osteopontin N-Half Assay Kit - IBL 96-Well $606.00
27259A Mouse Osteopontin N-Half Assay Kit - IBL 96-Well $606.00
27360A Rat Osteopontin Assay Kit - IBL 96-Well $606.00
 

 

 
 

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