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Cell Biology & Epigenetics


Products >  Cell_Biology_and_Epigenetics >  Cancer_and_Inflammation >  Mesothelin_ERC

Mesothelin/ERC Detection

The MSLN gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor (MPF) and mesothelin. Mesothelin is a glycosylphosphatidylinositol (GPI)-anchored cell-surface protein that may function as a cell-adhesion protein. Mesothelin is cleaved by a furin-like protease to yield fragments of approximately 31 kDa and 40 kDa. In humans, mesothelin is overexpressed in epithelial mesotheliomas, ovarian cancers, and in some types of squamous cell carcinomas. The ERC (expressed in renal carcinoma) gene, which was identified in a rat model of renal cancer, has been proposed as a homolog of the human MSLN gene. It plays a role in cell adhesion and shape. Rat mesothelin was also shown to be dynamically expressed in the developing pancreas. In rat and mouse, mesothelin is also known as ERC/mesothelin, protein expressed in renal carcinoma, and pre-pro-megakaryocyte-potentiating factor.

ELISAs for Mesothelin/ERC Detection

These kits are solid-phase sandwich ELISAs that include two antibodies that are highly specific to the 31-kDa fragment of human, rat, or mouse mesothelin (N-ERC); one is precoated on the ELISA plate and the other is HRP-conjugated. These assay kits can be used to measure soluble human, rat, or mouse mesothelin in serum, EDTA plasma, and cell culture supernatant samples.

Antibodies for Mesothelin/ERC Detection

These products are affinity-purified IgG antibodies that recognizes the C-ERC/Mesothelin (40-kDa fragment) of human, mouse, and rat mesothelin protein. The antibodies were raised in rabbit using synthetic peptides, and can be used for Western blot (WB) detection, immunohistochemical (IHC) detection, or flow cytometry (FCM) analysis of mesothelin.

  At-A-Glance   Documents   Images & Data

Features

Antigen
Cat. #
Application
Species
(Clone) and Source
Cross‑Reactivity
N-ERC/
Mesothelin
27765AELISARatCapture:
(30F2)
Mouse IgG MoAb

Detect: HRP (280)
Rabbit IgG
≤0.1% cross‑reactivity with rat MCP-1, TNF-alpha, and osteopontin
N-ERC/
Mesothelin
27783AELISAMouseCapture:
(150)
Rabbit IgG

Detect: HRP (280)
Rabbit IgG
<0.1% cross-reactivity with mouse C-ERC/Mesothelin
N-ERC/
Mesothelin
27190AELISAHumanCapture:
(7E7)
Mouse IgG MoAb

Detect: HRP (20A2)
Mouse IgG MoAb
<0.1% cross-reactivity with human C-ERC/Mesothelin and mouse N-ERC/Mesothelin
C-ERC/
Mesothelin
28001A, BIHC
WB
Rat(306)
Rabbit IgG
n/a
C-ERC/
Mesothelin
28127A, BIHC
WB
Mouse(308)
Rabbit IgG
n/a
C-ERC/
Mesothelin
10357A, BIHC
WB
FCM
Human(22A31)
Mouse IgG MoAb
n/a

Additional Information

Please see a product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.


 
 
Products
New Cat. # Product Contents Size Price Units Select
  28001A Anti-Rat C-ERC/Mesothelin (306) Rabbit IgG Affinity Purify 10 ug $113.00
  28001B Anti-Rat C-ERC/Mesothelin (306) Rabbit IgG Affinity Purify 100 ug $499.00
  27765A Rat N-ERC/Mesothelin Assay Kit - IBL 96-Well $606.00
  27783A Mouse N-ERC/Mesothelin Assay Kit - IBL 96-Well $849.00
  28127A Anti-Mouse C-ERC/Mesothelin (308) Rabbit IgG Affinity Purify 5 ug $114.00
  28127B Anti-Mouse C-ERC/Mesothelin (308) Rabbit IgG Affinity Purify 50 ug $525.00
  27190A Human N-ERC/Mesothelin Assay Kit - IBL 96-Well $930.00
10357A Anti-Human C-ERC/Mesothelin (22A31) Mouse IgG MoAb 10 ug $108.00
10357B Anti-Human C-ERC/Mesothelin (22A31) Mouse IgG MoAb 100 ug $445.00
 

 

 
 

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