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Products >  Cell_Biology_and_Epigenetics >  Cancer_and_Inflammation >  Hepatocyte_Growth_Factor

Hepatocyte Growth Factor Detection

Hepatocyte growth factor (HGF) belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. HGF is secreted by mesenchymal cells as a single inactive polypeptide that is cleaved by a serine protease (known as hepatocyte growth factor activator, or HGFA) into a 69-kDa alpha chain and 34-kDa beta chain. The active form of HGF is a heterodimer, produced by disulfide bond formation between the alpha and beta chains. HGF acts as a multifunctional cytokine in epithelial cells. It regulates cell growth, motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the c-Met receptor. HGF plays a role in tissue regeneration, angiogenesis, and tumorigenesis.

HGF is also known as hepapoietin A, hepatopoeitin A, scatter factor (SF), fibroblast-derived tumor cytotoxic factor (F-TCF), HGFB, HPTA, and deafness, autosomal recessive 39 (DFNB39).

ELISAs for Hepatocyte Growth Factor Detection

This kit is a solid-phase sandwich ELISA using two antibodies that are highly specific to human total HGF protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This assay kit can be used to measure soluble human total HGF protein in serum, EDTA plasma, or cell culture supernatants.

Antibodies for Hepatocyte Growth Factor Detection

These products are affinity-purified IgG antibodies that recognize human or rat HGF protein (alpha chain). The antibodies were raised in rabbit using synthetic peptides and can be used for Western blot (WB) detection or immunohistochemical (IHC) detection.

  At-A-Glance   Documents   Images & Data

Features

Antigen
Cat. #
Application
Species
(Clone) and Source
Cross‑Reactivity
Total HGF27402AELISAHumanCapture:
(n/a)
Rabbit IgG

Detect: HRP (n/a)
Goat IgG
≤0.1% cross‑reactivity with human HAI-1, HAI-2, c-Met, and HGFA
HGF alpha (N‑terminus)18281A, BIHC
WB
Rat(H56)
Rabbit IgG
Does not cross-react with rat HGF beta chain or human HGF alpha/beta
HGF alpha (N‑terminus)18131A, BIHC
WB
Human(H55)
Rabbit IgG
Reacts with rat HGF alpha

Does not cross-react with human HGF beta
HGF beta (C‑terminus)18132A, BIHC
WB
Human(H714)
Rabbit IgG
Does not cross-react with rat HGF
HGF alpha18133AIHC
WB
Human(H163)
Rabbit IgG
Cross-reacts with rat HGF alpha

Does not cross-react with human HGF beta
HGF beta (N‑terminus)18134A, BIHC
WB
Human(H495)
Rabbit IgG
Cross-reacts with rat HGF beta

Does not cross-react with human HGF alpha
HGF alpha (C‑terminus)18135A, BIHC
WB
Human(H487)
Rabbit IgG
Cross-reacts with rat HGF alpha

Does not cross-react with human HGF beta

Additional Information

Please see a product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price Units Select
27402A Human Total HGF Assay Kit - MCM 96-Well $1,924.00
18281A Anti-Rat HGF alpha(H56) Rabbit IgG Affinity Purify 10 ug $117.00
18281B Anti-Rat HGF alpha(H56) Rabbit IgG Affinity Purify 100 ug $514.00
18131A Anti-Human HGF alpha(H55) Rabbit IgG Affinity Purify 10 ug $117.00
18131B Anti-Human HGF alpha(H55) Rabbit IgG Affinity Purify 100 ug $514.00
18132A Anti-Human HGF beta (H714) Rabbit IgG Affinity Purify 10 ug $117.00
18132B Anti-Human HGF beta (H714) Rabbit IgG Affinity Purify 100 ug $514.00
18133A Anti-Human HGF alpha(H163) Rabbit IgG Affinity Purify 100 ug $514.00
18134A Anti-Human HGF beta (H495) Rabbit IgG Affinity Purify 10 ug $117.00
18134B Anti-Human HGF beta (H495) Rabbit IgG Affinity Purify 100 ug $514.00
18135A Anti-Human HGF alpha(H487) Rabbit IgG Affinity Purify 10 ug $117.00
18135B Anti-Human HGF alpha(H487) Rabbit IgG Affinity Purify 100 ug $514.00
 

 

 
 

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