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Products >  Cell_Biology_and_Epigenetics >  Cancer_and_Inflammation >  HB-EGF

Human HB-EGF Assay Kit

Heparin-binding EGF-like growth factor (HB-EGF) is a glycoprotein member of the EGF family and is a ligand for the ErbB family of tyrosine kinase receptors. HB-EGF is expressed in epidermal cells, cardiac myocytes, vascular endothelial cells, smooth myocytes, and macrophages. It is secreted into the blood and is involved in cell proliferation, differentiation, and inflammatory responses. Recently, it has been proposed that over-expression of HB-EGF plays a vital role in cancer invasion and metastasis, making HB-EGF an attractive drug target.

HB-EGF exists in 3 forms: a membrane-anchored form (proHB-EGF); soluble HB-EGF (sHB-EGF), which is the N-terminal fragment of proHB-EGF that has been separated by proteolytic cleavage at the cell surface; and the C terminal fragment (HB-EGF-CTF), which remains anchored to the cell membrane after cleavage. This solid phase sandwich ELISA kit uses 2 kinds of highly specific antibodies to quantify human HB-EGF in serum, EDTA plasma, urine, cerebrospinal fluid (CSF), and cell culture supernatants.

  At-A-Glance   Documents

Features

  • ELISA kit for the quantitative detection of human HB-EGF.

Applications

  • Measurement of HB-EGF in serum, EDTA plasma, urine, and CSF samples, and in cell culture supernatant.

Components

Precoated plate: Anti-Human HB-EGF (45F1) Mouse IgG MoAb96 well x 1
Labeled antibody (30X): HRP conjugated Anti-Human HB-EGF (58B6) Mouse IgG Fab’0.4 ml x 1
Standard: Recombinant Human HB-EGF0.5 ml x 2
EIA buffer: 1% BSA, 0.05% Tween-20 in PBS30 ml x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween-20 in PBS12 ml x 1
Chromogen: TMB solution15 ml x 1
Stop solution : 1N H2SO412 ml x 1
Wash buffer (40X): Phosphate buffer50 ml x 1

Storage

  • 2–8°C

Performance

  • Range of assay: 0.34–22 nmol/l
  • Sensitivity: 0.048 nmol/l
  • Assay duration: primary reaction—overnight; secondary reaction—60 minutes
  • Test specimen type: Serum, EDTA plasma, urine, cerebrospinal fluid, and cell culture supernatants
  • Sample volume per well: 100 µl

 
 
Products
Cat. # Product Contents Size Price Units Select
27189A Human HB-EGF Assay Kit - IBL 96-Well $981.00
 

 

 
 

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