Galectin-3 belongs to a family of carbohydrate-binding proteins known as lectins. In humans, it is encoded by the LGALS3 gene and is approximately 30 kDa in size. Galectin-3 has an N-terminal proline-rich tandem repeat domain, which allows it to self-associate, and a single C-terminal carbohydrate recognition domain. It binds beta-galactoside and IgE, and it is required for terminal differentiation of columnar epithelial cells during early embryogenesis. Galectin-3 is involved in a broad range of functions, including innate immunity, chemoattraction, cell adhesion, cell growth and differentiation, T-cell regulation, and apoptosis. Galectin-3 expression is implicated in processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and ventricular remodeling. Elevated levels of galectin-3 are also associated with cancer and inflammation.
Galectin-3 is also known as lectin, galactoside-binding, soluble, 3; carbohydrate-binding protein 35; galactose-specific lectin 3; laminin-binding protein; lectin L-29; Mac-2 antigen; IgE-binding protein; L31; GAL3; MAC2; CBP35; GALBP; GALIG; and LGALS2.
ELISAs for Galectin-3 Detection
This kit is a solid-phase sandwich ELISA using two antibodies that are highly specific to human galectin-3 protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This assay kit can be used to measure soluble human galectin-3 protein in serum, EDTA plasma, cell and tissue lysates, and cell culture supernatants.
Antibodies for Galectin-3 Detection
These products are affinity-purified IgG antibodies that recognize human galectin-3 protein. The antibodies were raised in mouse using recombinant protein, and can be used for Western blot (WB) detection, immunohistochemical (IHC) detection, or flow cytometry (FCM) of human galectin-3 protein.