Cyclooxygenase (COX) is a membrane-bound enzyme that exists as two different isoforms: a constitutively expressed form (COX-1) and an inducible form (COX-2).
COX-1 is also known as prostaglandin endoperoxide H synthase 1 (PHS-1 or PGHS-1), PTGS1, COX-3, PHS I, and PES-1. It converts arachidonic acid to prostaglandin endoperoxide H2 in a two-step reaction. COX-1 regulates angiogenesis in endothelial cells, and it is involved in cell signaling and maintaining tissue homeostasis.
COX-2 is also known as prostaglandin endoperoxide H synthase 2 (PHS-2 or PGHS-2), PTGS2, PHS II, GRIPGHS, and Tis10. It converts arachidonic acid to prostaglandin endoperoxide H2 in a two-step reaction. Levels of COX-2 are elevated during inflammation. COX-2 expression is also upregulated in many forms of cancer.
Nonsteroidal anti-inflammatory drugs (NSAIDs) inhibit prostaglandin production by both COX-1 and COX-2.
ELISAs for COX-2 Detection
The COX-2 Assay is a solid-phase sandwich ELISA that uses two highly specific antibodies to human or rat COX-2 protein; one antibody is precoated onto the COX-2 assay plate and the other antibody is HRP-conjugated. The COX-2 ELISA can be used to measure soluble human or rat COX-2 protein in cell culture lysates.
Antibodies for COX-1 and COX-2 Detection
These products are affinity-purified IgG antibodies that recognize human or rat COX-1 or COX-2. The antibodies were raised in rabbit or mouse using synthetic peptides, and can be used for Western blot (WB) detection, immunohistochemical (IHC) detection, or immunoprecipitation (IP) of human or rat COX-1 or COX-2 protein.