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Cell Biology & Epigenetics


Products >  Cell_Biology_and_Epigenetics >  Cancer_and_Inflammation >  COX-1_COX-2

COX-1 and COX-2 Detection

Cyclooxygenase (COX) is a membrane-bound enzyme that exists as two different isoforms: a constitutively expressed form (COX-1) and an inducible form (COX-2).

COX-1 is also known as prostaglandin endoperoxide H synthase 1 (PHS-1 or PGHS-1), PTGS1, COX-3, PHS I, and PES-1. It converts arachidonic acid to prostaglandin endoperoxide H2 in a two-step reaction. COX-1 regulates angiogenesis in endothelial cells, and it is involved in cell signaling and maintaining tissue homeostasis.

COX-2 is also known as prostaglandin endoperoxide H synthase 2 (PHS-2 or PGHS-2), PTGS2, PHS II, GRIPGHS, and Tis10. It converts arachidonic acid to prostaglandin endoperoxide H2 in a two-step reaction. Levels of COX-2 are elevated during inflammation. COX-2 expression is also upregulated in many forms of cancer.

Nonsteroidal anti-inflammatory drugs (NSAIDs) inhibit prostaglandin production by both COX-1 and COX-2.

ELISAs for COX-2 Detection

The COX-2 Assay is a solid-phase sandwich ELISA that uses two highly specific antibodies to human or rat COX-2 protein; one antibody is precoated onto the COX-2 assay plate and the other antibody is HRP-conjugated. The COX-2 ELISA can be used to measure soluble human or rat COX-2 protein in cell culture lysates.

Antibodies for COX-1 and COX-2 Detection

These products are affinity-purified IgG antibodies that recognize human or rat COX-1 or COX-2. The antibodies were raised in rabbit or mouse using synthetic peptides, and can be used for Western blot (WB) detection, immunohistochemical (IHC) detection, or immunoprecipitation (IP) of human or rat COX-1 or COX-2 protein.

  At-A-Glance   Documents   Images & Data

Features

Antigen
Cat. #
Application
Species
(Clone) and Source
Cross‑Reactivity
COX-227186AELISAHumanCapture:
(13H14)
Mouse IgG MoAb

Detect: HRP (n/a)
Rabbit IgG
n/a
COX-227187AELISARatCapture:
(C)
Rabbit IgG

Detect: HRP (2h3)
Mouse IgG MoAb
n/a
COX-118511A, BWBHuman(C24)
Rabbit IgG
Does not cross‑react with human COX-2 or rat COX-1
COX-118521A, BIHC
WB
Rat(C448)
Rabbit IgG
Does not cross-react with rat COX-2 or human COX-1
COX-210211A, BIHC
WB
IP
Human(13H14)
Mouse IgG1 MoAb
Does not cross-react with human COX-1
COX-218515A, BIHC
WB
Human(C295)
Rabbit IgG
Does not cross-react with human COX-1 or rat COX-2.
COX-218516A, BIHC
WB
Human(C631)
Rabbit IgG
Does not cross-react with human COX-1 or rat COX-2.
COX-218525A, BWBRat(C580)
Rabbit IgG
Does not cross-react with human COX-2 or rat COX-1.
COX-218955AIHC
WB
Rat(n/a)
Rabbit IgG
n/a

Additional Information

Please see a product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price Units Select
27186A Human COX-2 Assay Kit - IBL 96-Well $606.00
27187A Rat COX-2 Assay Kit - IBL 96-Well $606.00
10211A Anti-Human COX-2 (13H14) Mouse IgG MoAb 10 ug $113.00
10211B Anti-Human COX-2 (13H14) Mouse IgG MoAb 100 ug $499.00
18511A Anti-Human COX-1 (C24) Rabbit IgG Affinity Purify 10 ug $113.00
18511B Anti-Human COX-1 (C24) Rabbit IgG Affinity Purify 100 ug $499.00
18515A Anti-Human COX-2 (C295) Rabbit IgG Affinity Purify 10 ug $113.00
18515B Anti-Human COX-2 (C295) Rabbit IgG Affinity Purify 100 ug $499.00
18516A Anti-Human COX-2 (C631) Rabbit IgG Affinity Purify 10 ug $113.00
18516B Anti-Human COX-2 (C631) Rabbit IgG Affinity Purify 100 ug $499.00
18521A Anti-Rat COX-1 (C448) Rabbit IgG Affinity Purify 10 ug $113.00
18521B Anti-Rat COX-1 (C448) Rabbit IgG Affinity Purify 100 ug $499.00
18525A Anti-Rat COX-2 (C580) Rabbit IgG Affinity Purify 10 ug $113.00
18525B Anti-Rat COX-2 (C580) Rabbit IgG Affinity Purify 100 ug $499.00
18955A Anti-Rat COX-2 Rabbit IgG Affinity Purify 100 ug $499.00
 

 

 
 

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