DOCK-C (also called Zir) is a subfamily of the dedicator of cytokinesis (DOCK) family of guanine nucleotide exchange factors. Its members activate Rho-family small GTPases. Dock6, Dock7, and Dock8 were originally identified based on high sequence similarity to Dock180, the archetypal member of the DOCK family. They have a DHR1 domain that binds phospholipids and a DHR2 domain containing the guanine nucleotide exchange activity.
The cellular functions of Dock6 and Dock8 are largely unknown. Dock6 is reported to exhibit dual guanine nucleotide exchange specificity towards the small GTPases Rac1 and Cdc42. Mutations in the DOCK6 gene are associated with Adams-Oliver syndrome 2, a rare congenital disorder characterized by defects of the scalp, cranium, and limbs, and mottling of the skin. Mutations in the DOCK8 gene have been reported in a human lung cancer cell line, and Dock8 deficiency is associated with a variant of combined immunodeficiency, known as Hyperimmunoglobulin E syndrome (HIES). Dock6 alternative names include dedicator of cytokinesis protein 6, AOS2, ZIR1, and KIAA1395. Dock8 alternative names include dedicator of cytokinesis protein 8, ZIR8, MRD2, FLJ00026, FLJ00152, FLJ00346, and 1200017A24Rik.
Dock7 expression has been reported in neurons and in HEK 293 cells. It binds the small GTPases Rac1 and Rac3, but does not bind Cdc42. Dock7 is required for proliferation and differentiation of neurocytes and glial cells. Dock7 has also been reported to interact with the hamartin-tuberin complex, which is disrupted in patients with tuberous sclerosis. Alternative names for Dock7 include dedicator of cytokinesis protein 7, ZIR2, and KIAA1771
Antibodies for DOCK Detection
These products are affinity-purified IgG antibodies that recognize human Dock6, Dock7, or Dock8 protein. The antibodies were raised in rabbit using synthetic peptides and can be used for Western blot (WB) detection, immunocytochemical (ICC) detection, or immunoprecipitation (IP).