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Cell Biology & Epigenetics


Products >  Cell_Biology_and_Epigenetics >  Brain_and_CNS >  DOCK

DOCK Protein Detection

DOCK-C (also called Zir) is a subfamily of the dedicator of cytokinesis (DOCK) family of guanine nucleotide exchange factors. Its members activate Rho-family small GTPases. Dock6, Dock7, and Dock8 were originally identified based on high sequence similarity to Dock180, the archetypal member of the DOCK family. They have a DHR1 domain that binds phospholipids and a DHR2 domain containing the guanine nucleotide exchange activity.

The cellular functions of Dock6 and Dock8 are largely unknown. Dock6 is reported to exhibit dual guanine nucleotide exchange specificity towards the small GTPases Rac1 and Cdc42. Mutations in the DOCK6 gene are associated with Adams-Oliver syndrome 2, a rare congenital disorder characterized by defects of the scalp, cranium, and limbs, and mottling of the skin. Mutations in the DOCK8 gene have been reported in a human lung cancer cell line, and Dock8 deficiency is associated with a variant of combined immunodeficiency, known as Hyperimmunoglobulin E syndrome (HIES). Dock6 alternative names include dedicator of cytokinesis protein 6, AOS2, ZIR1, and KIAA1395. Dock8 alternative names include dedicator of cytokinesis protein 8, ZIR8, MRD2, FLJ00026, FLJ00152, FLJ00346, and 1200017A24Rik.

Dock7 expression has been reported in neurons and in HEK 293 cells. It binds the small GTPases Rac1 and Rac3, but does not bind Cdc42. Dock7 is required for proliferation and differentiation of neurocytes and glial cells. Dock7 has also been reported to interact with the hamartin-tuberin complex, which is disrupted in patients with tuberous sclerosis. Alternative names for Dock7 include dedicator of cytokinesis protein 7, ZIR2, and KIAA1771

Antibodies for DOCK Detection

These products are affinity-purified IgG antibodies that recognize human Dock6, Dock7, or Dock8 protein. The antibodies were raised in rabbit using synthetic peptides and can be used for Western blot (WB) detection, immunocytochemical (ICC) detection, or immunoprecipitation (IP).

  At-A-Glance   Documents   Images & Data

Features

Antigen/Peptide
Cat. #
Application
Species
(Clone) and Source
Cross‑Reactivity
Dock628111A, BICC
WB
IP
Human(n/a)
Rabbit IgG 
Cross-reacts with rat and mouse Dock6
Dock7 28057A, BICC
WB
IP
Human(n/a)
Rabbit IgG
Cross‑reacts with rat Dock7
Dock7 (phosphorylated)28079A, BWB
Human(Y1118)
Rabbit IgG 
Cross-reacts with rat and mouse Dock7
Dock828113A, BICC
WB
IP
Human(n/a)
Rabbit IgG
Cross-reacts with rat and mouse Dock8

 

Additional Information

Please see a product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price Units Select
28111A Anti-Dock6 Rabbit IgG Affinity Purify 10 ug $113.00
28111B Anti-Dock6 Rabbit IgG Affinity Purify 100 ug $499.00
28113A Anti-Dock8 Rabbit IgG Affinity Purify 10 ug $113.00
28113B Anti-Dock8 Rabbit IgG Affinity Purify 100 ug $499.00
28057A Anti-Human Dock7 Rabbit IgG Affinity Purify 5 ug $113.00
28057B Anti-Human Dock7 Rabbit IgG Affinity Purify 50 ug $499.00
28079A Anti-Human Dock7 (Y1118 Phosphorylated) Rabbit IgG Affinity Purify 5 ug $113.00
28079B Anti-Human Dock7 (Y1118 Phosphorylated) Rabbit IgG Affinity Purify 50 ug $499.00
 

 

 
 

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