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Cell Biology & Epigenetics


Products >  Cell_Biology_and_Epigenetics >  Brain_and_CNS >  14-3-3

Human 14-3-3 Family Detection

The 14-3-3 family of conserved regulatory proteins is expressed in all eukaryotic cells and its members are encoded by seven genes in mammals. 14-3-3 proteins bind many types of signaling proteins, including kinases, phosphatases, and transmembrane receptors. More than 100 signaling proteins have been reported as 14-3-3 ligands. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. Elevated amounts of 14-3-3 proteins are found in the cerebrospinal fluid of patients with Creutzfeldt-Jakob disease.

Antibodies for Human 14-3-3 Family Detection

14-3-3-beta
This isoform interacts with RAF1 and CDC25 phosphatases, suggesting a role in linking mitogenic signaling and the cell cycle machinery. Anti-Human 14-3-3-Beta Protein Rabbit IgG Antibody products (Cat. # 18641A, B) are affinity-purified IgG antibodies that recognize human 14-3-3-beta protein. The antibody was raised in rabbit using a synthetic peptide and can be used for Western blot (WB) detection of human 14-3-3-beta protein.

14-3-3-epsilon
This isoform interacts with CDC25 phosphatases, RAF1, and insulin receptor substrate 1 (IRS1), suggesting diverse functions related to signal transduction, e.g., cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Anti-Human 14-3-3-Epsilon Protein Rabbit IgG Antibody products (Cat. # 18643A, B) are affinity-purified IgG antibodies that recognize human 14-3-3-epsilon protein. The antibody was raised in rabbit using a synthetic peptide and can be used for Western blot (WB) detection or immunohistochemical (IHC) detection of human 14-3-3-epsilon protein.

14-3-3-eta
This isoform shows 99% similarity to the mouse, rat, and bovine orthologs. 14-3-3-eta is produced from the YWHAH gene, which contains a 7-bp repeat in its 5' UTR; changes in the number of this repeat have been associated with early-onset schizophrenia. Anti-Human 14-3-3-Eta Protein Rabbit IgG Antibody products (Cat. # 18645A, B) are affinity-purified IgG antibodies that recognize human 14-3-3-eta protein. The antibody was raised in rabbit using a synthetic peptide and can be used for Western blot (WB) detection or immunohistochemical (IHC) detection of human 14-3-3-eta protein.

14-3-3-gamma
Identical to the rat ortholog, 14-3-3-gamma expression is induced by growth factors in human vascular smooth muscle cells, and is elevated in skeletal and heart muscles. It interacts with RAF1, a serine/threonine protein kinase, and protein kinase C. Anti-Human 14-3-3-Gamma Protein Antibody products are affinity-purified IgG antibodies that recognize human 14-3-3-gamma protein. The antibodies were raised in rabbit (Cat. # 18647A, B) or mouse (Cat. # 10037A, B) using synthetic peptides and can be used for Western blot (WB) detection or immunohistochemical (IHC) detection of human 14-3-3-gamma protein.

14-3-3
Members of this family play diverse roles, including cell signaling, cell cycle regulation, and regulation of insulin sensitivity. Anti-Human 14-3-3 Protein Rabbit IgG Antibody (Cat. # 18649A, B) is an affinity-purified IgG antibody that recognizes human 14-3-3 proteins. The antibody was raised in rabbit using a synthetic peptide and can be used for Western blot (WB) detection or immunohistochemical (IHC) detection of human 14-3-3 proteins.

14-3-3-sigma
Also known as stratifin, 14-3-3-sigma is regulated by p53 and plays a role in the G2-M-phase checkpoint. Anti-Human 14-3-3-Sigma Rabbit IgG Antibody products (Cat. # 18640A, B, 18646A, B, and 18642A, B) are affinity-purified IgG antibodies that recognize human 14-3-3-sigma protein. The antibodies were raised in rabbit using synthetic peptides and can be used for Western blot (WB) detection or immunohistochemical (IHC) detection of human 14-3-3-sigma protein.

14-3-3-tau
14-3-3-tau (theta) is expressed at high levels in patients with amyotrophic lateral sclerosis (ALS), also called Lou Gehrig's disease. 14-3-3 Tau Monoclonal Antibody is an affinity-purified IgG antibody that recognizes human 14-3-3-tau (Cat. # 10017) proteins. The antibody was raised in mouse (Cat. # 10017A, B) using recombinant protein and can be used for Western blot (WB) detection or immunohistochemical (IHC) detection of human 14-3-3-tau. This antibody shows <6.25% reactivity with 14-3-3 sigma and therefore may be suitable for detection of this isoform.

14-3-3-zeta/delta
14-3-3-zeta/delta interacts with insulin receptor substrate 1 (IRS1), suggesting a role in regulating insulin sensitivity. Several transcript variants have been identified for the YWHAZ gene; they differ in the 5' UTR but encode the same protein. Anti-Human 14-3-3-Zeta Protein Rabbit IgG Antibody products (Cat. # 18644A, B) are affinity-purified IgG antibodies that recognize human 14-3-3-zeta protein. The antibody was raised in rabbit using a synthetic peptide and can be used for Western blot (WB) detection or immunohistochemical (IHC) detection of human 14-3-3-zeta protein.

  At-A-Glance   Documents   Images & Data

Features

Antigen/Peptide
Cat. #
Application
Species
(Clone) and Source
Cross‑Reactivity
14-3-3 beta
(N-terminus)
18641A, BWBHuman(n/a)
Rabbit IgG 
Does not cross‑react with other 14-3-3 isoforms
14-3-3 epsilon (C-terminus)18643A, BIHC
WB
Human(n/a)
Rabbit IgG 
Does not cross‑react with other 14-3-3 isoforms
14-3-3 eta
(N-terminus)
18645A, B IHC
WB
Human(n/a)
Rabbit IgG 
Does not cross‑react with other 14-3-3 isoforms
14-3-3 gamma (C-terminus) 10037A, B
18647A, B 
IHC
WB
Human(63A1)
Mouse IgG MoAb

(n/a) Rabbit IgG 
Does not cross‑react with other 14-3-3 isoforms
14-3-3 family18649A, BIHC
WB
Human(n/a)
Rabbit IgG 
Cross-reacts with human 14-3-3-beta, -gamma, -epsilon, -zeta, eta, and -tau proteins
14-3-3 sigma (N-terminus [18646A, B] and C-terminus [18642A, B])18646A, B
18642A, B
IHC
WB
Human(n/a)
Rabbit IgG
Does not cross‑react with other 14-3-3 isoforms
14-3-3 sigma 18640A, BIHC
WB
Human(69)
Rabbit IgG
Does not cross‑react with other 14-3-3 isoforms
14-3-3 tau (recombinant protein expressed in Sf21 cells)10017A, BIHC
WB
Human(33A)
Mouse IgG MoAb
Shows 0.1%-0.4% cross-reactivity with other 14-3-3 proteins and <6.25% cross-reactivity with 14-3-3-sigma
14-3-3 zeta
(N-terminus)
18644A, BIHC
WB
Human(n/a)
Rabbit IgG
Does not cross‑react with other 14-3-3 isoforms

 

Additional Information

Please see a product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price Units Select
18641A Anti-Human 14-3-3 beta Protein Rabbit IgG Affinity Purify 10 ug $113.00
18641B Anti-Human 14-3-3 beta Protein Rabbit IgG Affinity Purify 100 ug $499.00
10037A Anti-Human 14-3-3 gamma Protein (63A1) Mouse IgG MoAb 10 ug $113.00
10037B Anti-Human 14-3-3 gamma Protein (63A1) Mouse IgG MoAb 100 ug $499.00
10017A Anti-Human 14-3-3 tau Protein (33A) Mouse IgG MoAb 10 ug $113.00
10017B Anti-Human 14-3-3 tau Protein (33A) Mouse IgG MoAb 200 ug $499.00
18640A Anti-Human 14-3-3 sigma Protein (69) Rabbit IgG Affinity Purify 10 ug $113.00
18640B Anti-Human 14-3-3 sigma Protein (69) Rabbit IgG Affinity Purify 100 ug $499.00
18646B Anti-Human 14-3-3 sigma Protein (N) Rabbit IgG Affinity Purify 100 ug $499.00
18647A Anti-Human 14-3-3 gamma Protein Rabbit IgG Affinity Purify 10 ug $113.00
18647B Anti-Human 14-3-3 gamma Protein Rabbit IgG Affinity Purify 100 ug $499.00
18642A Anti-Human 14-3-3 sigma Protein (C) Rabbit IgG Affinity Purify 10 ug $113.00
18649A Anti-Human 14-3-3 Protein Rabbit IgG Affinity Purify 10 ug $113.00
18649B Anti-Human 14-3-3 Protein Rabbit IgG Affinity Purify 100 ug $499.00
18642B Anti-Human 14-3-3 sigma Protein (C) Rabbit IgG Affinity Purify 100 ug $499.00
18643A Anti-Human 14-3-3 epsilon Protein Rabbit IgG Affinity Purify 10 ug $113.00
18643B Anti-Human 14-3-3 epsilon Protein Rabbit IgG Affinity Purify 100 ug $499.00
18644A Anti-Human 14-3-3 zeta Protein Rabbit IgG Affinity Purify 10 ug $113.00
18644B Anti-Human 14-3-3 zeta Protein Rabbit IgG Affinity Purify 100 ug $499.00
18645A Anti-Human 14-3-3 eta Protein Rabbit IgG Affinity Purify 10 ug $113.00
18645B Anti-Human 14-3-3 eta Protein Rabbit IgG Affinity Purify 100 ug $499.00
18646A Anti-Human 14-3-3 sigma Protein (N) Rabbit IgG Affinity Purify 10 ug $113.00
 

 

 
 

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