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Products >  Cell_Biology_and_Epigenetics >  Bone_Research >  Procollagen_Type_I_C-Peptide

Procollagen Type I C-Peptide (PIP) Detection

Collagen types I, II, III, IV, and V are synthesized as precursor molecules called procollagens. A procollagen peptide contains an additional peptide sequence, the propeptide, at its amino- and carboxy-terminal ends. Propeptides facilitate the winding of procollagen molecules into a triple-helix conformation within the endoplasmic reticulum. These propeptides are then cleaved from the collagen triple helix molecule during its secretion, after which the collagens polymerize into extracellular collagen fibrils. Thus, the amount of free propeptides directly correlates with collagen molecule synthesis. Procollagen Type I C-peptide (PIP) has been extensively referenced in correlation studies between collagen levels and certain health disorders such as bone disease, alcoholic liver disease, liver cirrhosis, and scirrhous (Borrmann type IV) adenocarcinoma of the stomach.

ELISA Kits for PIP Detection

The Procollagen Type I C-Peptide (PIP) EIA Kit (Precoated) is a 96-well format in vitro enzyme immunoassay intended for the quantification of human, bovine, canine, and horse PIP in plasma, serum, cell culture extract, cell culture supernatant, and other biological fluids. The kit is a solid-phase procollagen sandwich ELISA that utilizes two mouse monoclonal PIP antibodies; the capture antibody is coated onto the plate, while the detection antibody is peroxidase-labeled. The procollagen ELISA takes advantage of a one-step format in which test samples and peroxidase-labeled anti-PIP are simultaneously added to the wells of the plate and incubated. During incubation, PIP becomes bound to the antibody coating the plate and is tagged by peroxidase-labeled anti-PIP at its opposite side. A substrate (H2O2, TMBZ) is added and the reaction between peroxidase and substrate results in color development. The amount of sample PIP is determined by measuring absorbance using an EIA plate reader. Accurate PIP sample concentrations can be determined by comparing their specific absorbance with that obtained for the supplied kit standard (via a standard curve).

Antibodies for PIP Detection

Monoclonal Anti-Human Procollagen Type I C-Peptide Antibodies (Clone PC5-5 and Clone PC8-7) are suitable for monitoring collagen synthesis via Western blot (WB) detection of non-denatured antigen in cell culture supernatant as well as in FFPE and frozen tissue sections.

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Features

Antigen/Peptide
Cat. #
Application
Species
(Clone) and Source
Cross‑Reactivity
Procollagen Type I C-peptideMK101ELISAHumanCapture:
(n/a)
Mouse IgG MoAb

Detect: HRP
(n/a)
Mouse IgG MoAb
No cross-reactivity with fibronectin, vitronectin, laminin, collagen type I, or collagen type III

Cross‑reacts with bovine, canine, and horse PIP

No cross‑reactivity with mouse PIP; applications with other sources have not been tested

Note: Kit signal may be altered if used with samples containing animal-derived serums such as fetal bovine and horse serums; It is recommended that measurements be performed under serum-free conditions
Procollagen Type I C-peptideM011IHC
WB
Human(PC5-5) 
BALB/c Mouse IgG MoAb
Cross-reacts with bovine, horse, and dog procollagen

Does not react with rat or rabbit procollagen

Procollagen Type I C-peptideM012IHC
WB
Human(PC8-7)
BALB/c Mouse IgG MoAb
Cross-reacts with bovine, horse, and dog procollagen

Does not react with rat or rabbit procollagen

Additional Information

Please see a product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price Units Select
M011 Monoclonal Anti-Human Procollagen Type I C-Peptide (PIP) (Clone PC5-5) 0.1 mg $315.00
M012 Monoclonal Anti-Human Procollagen Type I C-Peptide (PIP) (Clone PC8-7) 0.1 mg $315.00
MK101 Procollagen Type I C-Peptide (PIP) EIA Kit 96 Assays $540.00
 

 

Recommended Products
Cat. # Product Contents Size Price Units Select
M011 Monoclonal Anti-Human Procollagen Type I C-Peptide (PIP) (Clone PC5-5) 0.1 mg $315.00
M012 Monoclonal Anti-Human Procollagen Type I C-Peptide (PIP) (Clone PC8-7) 0.1 mg $315.00
 
 
 

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