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Cell Biology & Epigenetics


Products >  Cell_Biology_and_Epigenetics >  Bone_Research >  Osteocalcin_Rat

Osteocalcin (Rat) Detection

Osteocalcin, which contains 2–3 gamma-carboxyglutamate (Gla) residues and has a molecular weight of approximately 5.9 kDa, is known as a vitamin K-dependent calcium-binding non-collagenous protein. Produced only by osteoblasts, osteocalcin has been used as a unique marker of bone formation and resorption. The rat osteocalcin protein consists of 50 amino acids, while human, bovine, rabbit and other species express an osteocalcin protein containing 49 amino acids. Osteocalcin is also a hormone that plays a role in insulin regulation and male fertility. Meanwhile, the undercarboxylated form of osteocalcin (ucOC) is responsible for glucose metabolism. The Gla-osteocalcin and Glu-osteocalcin ELISA kits enable researchers to study the roles of both carboxylated and undercarboxylated osteocalcin in metabolic disease, male fertility, and bone formation and resorption.

Bone formation and resorption require the action of osteoblasts, which produce osteocalcin exclusively. Osteocalcin is typically produced with all three of its glutamate residues carboxylated (Gla-OC), affording the protein the ability to form a calcium pocket and bind bone matrix. The carboxylated glutamate residues are at positions 17, 21, and 24 of the protein. During bone metabolism, osteocalcin is released from bone matrix through the action of various enzymes, including one produced by osteoclasts. Most of the three glutamate residues are decarboxylated on osteocalcin (Glu-OC) when it is released into bloodstream from bone. Thus, osteocalcin is present in blood in both its Gla and Glu forms.

Alternate names: bone gamma-carboxyglutamic acid-containing protein, BGLAP.

ELISA Kits for Osteocalcin (Rat) Detection

The Rat Gla-Osteocalcin High Sensitive EIA Kit (Cat. # MK126) is a 96-well format sandwich-type EIA that takes advantage of a C-terminal rat osteocalcin monoclonal antibody to capture osteocalcin (OC) in rat biological samples. This antibody has minimal cross-reactivity to bovine, human, and rabbit osteocalcin. An enzyme-labeled antibody (Gla-OC4-30) specific to Gla-OC is used as the detection antibody, allowing this kit to detect Gla-osteocalcin with very high sensitivity. As such, this EIA kit is sensitive enough to pick up even minute traces of rat Gla-osteocalcin in cell supernatants supplemented with fetal calf serum.

The Rat Glu-Osteocalcin High Sensitive EIA Kit (Cat. # MK146) is a 96-well format sandwich-type EIA that uses a C-terminal rat osteocalcin monoclonal antibody to capture osteocalcin (OC). This kit's detection antibody specifically recognizes the glutamate residues present at positions 21 and 24 of the protein, making highly sensitive yet reproducible measurements of low-level Glu-OC possible. Moreover, because both the Rat Gla-Osteocalcin High Sensitive EIA Kit (Cat. # MK126) and the Rat Glu-Osteocalcin High Sensitive EIA Kit (Cat. # MK146) contain the same capture antibody, both kits may be used for Gla/Glu detection (Cat. # MK147), making simultaneous monitoring of bone formation and resorption possible.

Antibodies for Osteocalcin (Rat) Detection

Monoclonal Anti-Rat Osteocalcin (Clone D-8G, Clone 6-7H, Clone 9-12H) antibodies selectively detect rat osteocalcin. Cat. # M185 and Cat. # M186 may be used for osteocalcin immunohistochemistry (IHC) with formalin-fixed and paraffin-embedded tissues, while Cat. # M186 and Cat. # M187 may be used for Western blot (WB) analysis.

  At-A-Glance   Documents   Images & Data

Features

Antigen/Peptide
Cat. #
Application
Species
(Clone) and Source
Cross‑Reactivity
Gla-Osteocalcin (C-terminus)MK126ELISARatCapture:
(n/a)
MoAb

Detect: HRP 
(GlaOC4-30) MoAb
Minimal cross‑reactivity with bovine, human, and rabbit osteocalcin
Glu-Osteocalcin (C-terminus)MK146ELISARatCapture:
(n/a)
MoAb 

Detect: HRP 
(n/a)
MoAb

Detection antibody epitope: Glu residues that straddle positions 21 and 24 of osteocalcin
Does not cross-react with bovine, human, horse, dog, pig, sheep, goat, rabbit, or guinea pig osteocalcin
Gla/Glu-Osteocalcin
(C-terminus)
MK147 (contains MK126 and MK146)ELISARatSee Clone and Source information for M126 and M146, above.Minimal cross-reactivity with bovine, human, and rabbit osteocalcin
Osteocalcin
(1-25) conjugated to KLH
M185IHCRat(D-8G)
BALB/c Mouse IgG1 MoAb
Cross‑reacts with bovine, pig, goat, sheep, and donkey osteocalcin

Does not react with human osteocalcin
Osteocalcin
(1-25) conjugated to KLH
M186IHC
WB

Rat(6-7H)
BALB/c Mouse IgG1 MoAb
Cross-reacts with bovine osteocalcin

Does not react with human osteocalcin
Osteocalcin
(38-50) conjugated to KLH
M187WB
Rat(9-12H)
BALB/c Mouse IgG1 MoAb
Cross-reacts with rat osteocalcin

Does not react with human and bovine osteocalcin

Additional Information

Please see a product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
MK147 Gla/Glu Osteocalcin High Sensitive EIA Set (Rat) Each $1,044.00  
M186 Monoclonal Anti-Rat Osteocalcin (Clone 6-7H) 0.1 mg $357.00  
M187 Monoclonal Anti-Rat Osteocalcin (Clone 9-12H) 0.1 mg $357.00 License Statements
M185 Monoclonal Anti-Rat Osteocalcin (Clone D-8G) 0.1 mg $357.00  
MK126 Rat Gla-Osteocalcin High Sensitive EIA Kit 96 Rxns $653.00  
MK146 Rat Glu-Osteocalcin High Sensitive EIA Kit 96 Rxns $653.00  
 

 

 
 

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