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Products >  Cell_Biology_and_Epigenetics >  Bone_Research >  Osteocalcin_Mouse

Osteocalcin (Mouse) Detection

Mouse osteocalcin is a vitamin K-dependent calcium-binding non-collagenous protein found in bone and dentin that consists of 49 amino acids. Produced only by osteoblasts, osteocalcin has been used as a unique marker of bone formation and resorption. Osteocalcin is also a hormone that plays a role in insulin regulation and male fertility. During bone metabolism, osteocalcin is released from bone matrix through the action of various enzymes, including one produced by osteoclasts. Unmodified Glu-type osteocalcin is produced by osteoblasts and is converted to active Gla-type osteocalcin; its binding to the bone matrix is enabled through the enzymatic conversion of glutamic acid to carboxyglutamate. Bone turnover can be analyzed by simultaneously measuring Gla-type and Glu-type osteocalcin. Most of the three glutamate residues are decarboxylated on osteocalcin (Glu-OC) when it is released into bloodstream from bone. Thus, osteocalcin is present in blood in both its Gla and Glu forms. Recent findings suggest that Glu-type osteocalcin may play an important role in the metabolism of sugar.

Alternate names: bone gamma-carboxyglutamic acid-containing protein, BGLAP.

ELISA Kits for Osteocalcin (Mouse) Detection

Gla-osteocalcin and Glu-osteocalcin can be assayed concurrently with the Mouse Gla-Osteocalcin High Sensitive EIA Kit (Cat. # MK127) and Mouse Glu-Osteocalcin High Sensitive EIA Kit (Cat. # MK129), respectively. These mouse osteocalcin ELISA kits allow for the relative evaluation of Gla/Glu-osteocalcins, thereby providing a measure of both bone formation and bone resorption.

These kits use a sandwich ELISA strategy to specifically detect the Gla and Glu forms of osteocalcin. The capture antibody is a plate-bound solid-phased rat monoclonal antibody that specifically recognizes the C-terminal region of mouse osteocalcin. This is paired with labeled monoclonal antibodies that are specific for captured Glu-type or Gla-type osteocalcin. Because mouse osteocalcin has C-terminal sequences that differ from other mammals, it is possible to measure mouse osteocalcin without any cross-reaction with bovine antigens. Therefore, the process of osteoblastic cell differentiation can be monitored in pluripotent cells, such as mouse ES and iPS cells, without interference from bovine serum included in the culture medium. Furthermore, these kits can be used for high-sensitivity measurements from a variety of samples, including cell culture supernatants, blood, or body fluids.

Antibodies for Osteocalcin (Mouse) Detection

Monoclonal Anti-Mouse Osteocalcin (Clone R21C-01A) was obtained by fusing mouse myeloma cell-line P3U1 with the spleen cells of Sprague Dawley rats following their immunization with mouse osteocalcin peptide (amino acids 25–46) conjugated to keyhole limpet hemocyanin (KLH). The osteocalcin antibody was harvested from severe combined immunodeficiency (SCID) mouse ascitic fluid.

Polyclonal Anti-Mouse Osteocalcin, Clone mOC(1–20) antibody was raised against an N-terminal mouse osteocalcin peptide (amino acids 1–20) and specifically recognizes mouse osteocalcin.

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Features

Antigen/Peptide
Cat. #
Application
Species
(Clone) and Source
Cross‑Reactivity
Gla-Osteocalcin (C-terminus)MK127ELISAMouseCapture:
(n/a)
Rat IgG MoAb

Detect: HRP
(n/a)
MoAb
Does not cross‑react with bovine antigens and can be used directly for cell culture supernatants using bovine serum-containing medium

Some cross-reactivity may be observed with rabbit osteocalcin*
Glu-Osteocalcin (C-terminus)MK129ELISAMouseCapture: 
(n/a)
Rat IgG MoAb

Detect: HRP
(n/a)
MoAb
Does not cross-react with bovine antigens and can be used directly for cell culture supernatants using bovine serum-containing medium.

Some cross‑reactivity may be observed with rabbit osteocalcin*
Osteocalcin
(25-46) conjugated to KLH
M188IHC
WB
Mouse(R21C-01A)
Rat IgG2a MoAb
Does not cross-react with bovine, rat, or human osteocalcin
Osteocalcin
(N-terminus
[1-20]) conjugated to KLH
M173IHCMouse(n/a)
Rabbit IgG PoAb
Does not cross-react with rat osteocalcin

* While some cross-reactivity is observed with rabbit samples, these assays are not suitable for quantitative measurement of rabbit osteocalcin. We recommend using the Gla-Osteocalcin EIA Kit (Cat. # MK111) for this application.

Additional Information

Please see a product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price Units Select
M188 Monoclonal Anti-Mouse Osteocalcin (Clone R21C-01A) 0.1 mg $357.00
MK127 Mouse Gla-Osteocalcin High Sensitive EIA Kit 96 Rxns $653.00
MK129 Mouse Glu-Osteocalcin High Sensitive EIA Kit 96 Rxns $893.00
M173 Polyclonal Anti-Mouse Osteocalcin (Clone mOC(1-20)) 0.1 mg $357.00
 

 

Recommended Products
Cat. # Product Contents Size Price License Units Select
MK111 Gla-Type Osteocalcin (Gla-OC) EIA Kit 96 Assays $713.00  
MK118 Undercarboxylated Osteocalcin (Glu-OC) EIA Kit 96 Rxns $713.00 License Statements
M041 Monoclonal Anti-Bovine Osteocalcin (Clone OC4-30) 0.1 mg $357.00  
M171 Monoclonal Anti-Human Undercarboxylated Osteocalcin (Clone GluOC4-5) 0.1 mg $357.00  
 
 
 

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