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Products >  Cell_Biology_and_Epigenetics >  Bone_Research >  Osteocalcin_Carboxylated_Gla-OC

Osteocalcin ELISA Kit: Gla-Type Osteocalcin (Gla-OC) EIA Kit

The Takara Bio Osteocalcin ELISA Kit offers sensitive detection of the osteogenic marker osteocalcin (OC). OC, also known as gamma-carboxyglutamic acid protein, is a small (5.9 kDa), vitamin K-dependent, hydroxyapatite (Ca2+)-binding protein synthesized exclusively by osteoblasts and odontoblasts. The tissue-specific expression of OC makes it an excellent osteogenic marker for measuring osteoblast activity during bone formation. Osteocalcin is also a hormone that controls the regulation of glucose and fat deposition and plays a role in male fertility. Three gamma-carboxyglutamic acid (Gla) residues at positions 17, 21, and 24 of the protein are responsible for binding calcium. OC calcium-binding is also required for such biological activities as activation of the blood coagulation cascade. Carboxylated OC (Gla-OC) is most likely the active form of the protein, while decarboxylated OC (Glu-OC), which has weak hydroxyapatite affinity, represents the inactive form. Gla-OC versus Glu-OC serum levels correlate well with bone formation; thus, the Takara Bio human osteocalcin ELISA kit provides a better analysis of bone growth and remodeling than conventional assays that do not differentiate between the active and inactive protein forms.

The Gla-Type Osteocalcin (Gla-OC) EIA Kit is a 96-well in vitro enzyme immunoassay kit for the quantitative determination of human Gla-OC in serum, cultured cell extract, cell culture supernatant and other biological fluids. It is a solid phase sandwich EIA utilizing two mouse monoclonal Gla-OC antibodies, one of which is coated onto the plate, and the other of which is peroxidase-labeled. This assay format permits highly sensitive detection of Gla-OC using the following two-step incubation method: In the first step, biological samples are incubated in the antibody-coated microtiter plate; in the second step, the plate is washed and incubated with the peroxidase-labeled Gla-OC antibody. A substrate is added and the reaction between the peroxidase and substrate (H2O2, TMBZ) results in color development. The amount of sample Gla-OC is determined by absorbance measurement using an EIA plate reader. Accurate Gla-OC sample concentration can be determined by comparing its specific absorbance with that of the supplied standard on a standard curve.

 
  At-A-Glance   Documents   Images & Data

Features

  • Human osteocalcin ELISA
  • Highly sensitive quantitative measurement of human Gla-OC

Applications

  • Quantitative measurement of human, bovine, rabbit, sheep, canine or goat carboxylated osteocalcin

Components

Antibody coated microtiter plate1 plate (8 wells x 12 strips)
Antibody-peroxidase conjugate (lyophilized) 1 vial (for 1 x 11 mL)
Standard1 vial (8 ng)
Sample diluent2 vials (2 x 11 mL)
Substrate solution 1 vial (1 x 12 mL)

Storage

2–8°C

Performance Characteristics

  • Range of assay: 0.5-16 ng/mL
  • Assay sensitivity: 0.5 ng/mL
  • Assay duration: 3.5 hours
  • Specificity: Human Gla-OC. No detectable cross-reactivity with Glu-OC.
  • Species cross-reactivity: In addition to human Gla-OC, also cross-reacts with bovine, rabbit, sheep, canine and goat Gla-OC. No reactivity with mouse and rat Gla-OC. Applications for other species have not been tested.
  • Test specimen type: Serum, plasma, culture supernatant and cell extract.
  • Sample volume per well: 100 µL

References

1. Tsukamoto, Y. et al. (2000) J. Bone Miner. Metab. 18(4):216-22.

2. Rubio-Gozalbo, M.E. et al. (2002) Arch. Dis. Child. 87(1):57-60.

3. Shimizu, N. et al. (2002) Pediatr. Res. 52(5):656-9.

4. Schurgers, L.J. et al. (2004) Blood 104(9):2682-2689. 

5. Conway, S.P. et al. (2005) J. Pediatrics 115(5):1325-1331.

6. Kaptoge, S. et al. (2007) J. Clin. Endocrinol. Metab. 92(1):304-313.

7. Barroga, E.F. et al. (2000) Vet. J. 159(2):186-193.

8. Nakamura, K. et al. (2005) Am. J. Clin. Nutr. 82(5):1127-1133.

9. Schurgers, L.J. et al. (2002) J. Lipid Res. 43(6):878-884.


 
 
Products
Cat. # Product Contents Size Price Units Select
MK111 Gla-Type Osteocalcin (Gla-OC) EIA Kit 96 Assays $776.00
 

 

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