Annexin V apoptosis assay
The ApoAlert Annexin V-FITC Apoptosis Kit provides a simple and effective method to detect one of the earliest events in apoptosis—the externalization of phosphatidylserine (PS)—in living cells. Soon after apoptosis is induced, PS is translocated from the inner leaflet of the plasma membrane to the outer leaflet. This assay uses annexin V, which has a strong and specific affinity for PS, to monitor the PS translocation that occurs due to apoptosis. Labeled annexin V provides a simple staining assay to monitor apoptosis by flow cytometry or fluorescence microscopy.
Overview
- Easy to use: non-enzymatic assay, no fixation
- Simple protocol: one-step procedure that requires just 10 min to perform
- Versatile: suitable for both adherent and suspension cells
More Information
Applications
- Early apoptosis detection
- Monitor phosphatidylserine translocation by flow cytometry or fluorescence microscopy
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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