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Products >  Cell_Biology_and_Epigenetics >  Apoptosis_Kits >  Glutathione_Detection_Assay

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Glutathione Detection Assay

Glutathione Detection │ Glutathione Assay

Applications

  • Use this glutathione assay to monitor the early stages of apoptosis

Features

  • Glutathione assay that monitors early stages of apoptosis by measuring redox change
  • Simple, quantitative in vitro assay
  • Easily adaptable to a 96-well format

The ApoAlert Glutathione Detection Kit is a quantitative in vitro assay for that detects decreased cytosolic glutathione (GSH) levels that occur early in apoptosis in some cell types. In healthy cells, glutathione acts as a redox buffer. However, in Jurkat and some other cell types, the plasma membrane contains an ATP-dependent GSH transport system that is triggered by the initiation of apoptosis. When GSH is actively pumped out of the cell, the cytosol is shifted from a reducing to an oxidizing environment. You can detect the drop in glutathione levels with this kit.

This glutathione assay includes a monochlorobimane (MCB) dye that fluoresces blue when bound to glutathione. It is easy to detect the decrease in glutathione levels in apoptotic cell extracts (compared to nonapoptotic cell extracts), using a fluorometer or 96-well fluorescence plate reader.

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