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Glutathione Detection Assay
Glutathione Detection │ Glutathione Assay
Use this glutathione assay to monitor the early stages of apoptosis
Glutathione assay that monitors early stages of apoptosis by measuring redox change
Simple, quantitative in vitro assay
Easily adaptable to a 96-well format
The ApoAlert Glutathione Detection Kit is a quantitative in vitro assay for that detects decreased cytosolic glutathione (GSH) levels that occur early in apoptosis in some cell types. In healthy cells, glutathione acts as a redox buffer. However, in Jurkat and some other cell types, the plasma membrane contains an ATP-dependent GSH transport system that is triggered by the initiation of apoptosis. When GSH is actively pumped out of the cell, the cytosol is shifted from a reducing to an oxidizing environment. You can detect the drop in glutathione levels with this kit.
This glutathione assay includes a monochlorobimane (MCB) dye that fluoresces blue when bound to glutathione. It is easy to detect the decrease in glutathione levels in apoptotic cell extracts (compared to nonapoptotic cell extracts), using a fluorometer or 96-well fluorescence plate reader.