DNA Fragmentation Assay

The ApoAlert DNA Fragmentation Assay Kit detects nuclear DNA fragmentation, a hallmark of apoptosis. The kit can be used with tissue sections as well as cultured cells, and results can be visualized directly by fluorescence microscopy or quantified by flow cytometry. During apoptosis, cellular endonucleases cleave nuclear DNA between nucleosomes, producing a mix of DNA fragments whose length varies in multiples of 180 to 200 bp. The ApoAlert DNA fragmentation assay incorporates fluorescein-dUTP at the free 3'-hydroxyl ends of the fragmented DNA using the TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick-end-labeling) method. The fluorescein-labeled DNA can then be quantified using either fluorescence microscopy or flow cytometry.

At-A-Glance

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Detect nuclear DNA fragmentation in situ
Use with adherent cells, suspension cells, or fixed tissue sections
Analyze results by fluorescence microscopy or flow cytometry

Products

Cat. #	Product	Package Size	Price	# of Units	Select

630105	ApoAlert™ Cell Fractionation Kit	100 Assays	$563.00	# of Units	Select

630108	ApoAlert™ DNA Fragmentation Assay Kit	100 Assays	$615.00	# of Units	Select


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DNA Fragmentation Assay

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