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Cell Biology & Epigenetics


Products >  Cell_Biology_and_Epigenetics >  Alzheimers_Disease >  sAPP

Soluble Amyloid Precursor Protein (sAPP) Detection

The accumulation of amyloid-beta protein in the cerebral cortex in Alzheimer's disease (AD) is only one of the outcomes of the proteolytic cleavage of amyloid precursor protein (APP). Cleavage by beta- and gamma-secretase (in the amyloidogenic pathway) leads to production of amyloid beta and soluble APP-beta (sAPP-beta), while cleavage by alpha-secretase (in the nonamyloidogenic pathway) results in another soluble form of APP, sAPP-alpha. Several mutations in the sAPP gene can give rise to variants of both sAPP-alpha and sAPP-beta, and more than 50 different mutations in the APP gene can cause early-onset Alzheimer’s disease.

A double missense mutation (Lys670→Asn and Met671→Leu) in APP found in a Swedish pedigree (APP-beta-sw) elevates Aβ40 and Aβ42 production, and the mutation is utilized in establishment of transgenic mice overexpressing a mutant form of human amyloid precursor protein. Amyloid beta production and beta-secretase cleavage of APP-beta-sw reportedly occur in the endoplasmic reticulum, Golgi, and endocytic compartments.

ELISAs for sAPP Detection

These kits are solid-phase sandwich ELISAs using two highly specific antibodies to sAPP variants; one is precoated on the ELISA plate and the other is HRP-conjugated. The human sAPP ELISA kit can be used to measure human sAPP variants in serum, EDTA plasma, cerebrospinal fluids, or cell culture supernatant. The rodent sAPP-alpha ELISA kit can be used to measure mouse or rat sAPP-alpha as well as full-length APP in serum, EDTA plasma, cell culture supernatant, and brain extract samples. The mouse sAPP-beta-w ELISA Kit can be used to measure mouse sAPP-beta-w in mouse plasma and cell culture supernatant.

Antibodies for sAPP Detection

These antibody products are affinity-purified IgG antibodies that recognize the C-terminus of human soluble APP-alpha (Cat. # 11088) and soluble APP-beta-Swedish (sAPP-beta-sw) (Cat. # 10321). The APP Antibody products are raised in mouse using synthetic peptides and can be applied to Western blot (WB) and immunohistochemical (IHC) detection (Cat. # 10321 only) as well as immunoprecipitation (IP)
(Cat. # 11088 only) of human sAPP-alpha and sAPP-beta-sw. The sAPP-beta antibody is an affinity-purified rabbit IgG antibody that recognizes wild-type (w) human soluble APP-beta protein. The sAPP-beta antibody is raised in rabbit using a synthetic peptide and can be applied to Western blot (WB) or immunoprecipitation (IP) of human sAPP-beta.

  At-A-Glance   Documents   Images & Data

Features

Antigen/Peptide
Cat. #
Application
Species
(Clone) and Source
Cross‑Reactivity
Total sAPP (includes sAPP-alpha, sAPP-beta-w, and sAPP-beta-sw)27731AELISAHumanCapture:
(R12A1)
Mouse IgG MoAb 

Detect: HRP (R101A4)
Mouse IgG MoAb
n/a
sAPP-beta-w 27732AELISAHumanCapture:
(n/a)
Rabbit IgG 

Detect: HRP (R101A4)
Mouse IgG MoAb
Low cross‑reactivity with sAPP-alpha/sAPP-beta-sw 
sAPP-beta-w 27416AELISAMouseCapture:
(n/a)
Rabbit IgG 

Detect: HRP 
Rabbit IgG MoAb
Low cross‑reactivity with mouse sAPP-alpha 
sAPP-beta-sw27733AELISAHumanCapture:
(n/a)
Rabbit IgG 

Detect: HRP (R101A4)
Mouse IgG MoAb
≦0.1 % cross-reactivity with human sAPP-alpha; 0.11% cross-reactivity with human sAPP-beta-w
sAPP-alpha27734AELISAHumanCapture:
(2B3)
Mouse IgG MoAb

Detect: HRP (R101A4)
Mouse IgG MoAb
≦0.1 % cross-reactivity with human sAPP-w and sAPP-beta-sw
sAPP-alpha
(N-terminus)
27419AELISA
Mouse RatCapture:
(599)
Rabbit IgG 

Detect: HRP (n/a)
Rabbit IgG
0.15% cross‑reactivity with sAPP-beta isoforms

Cross-reacts with full-length APP
sAPP-beta-sw 
(C-terminus)
10321A, BIHC
WB
Human(6A1)
Mouse IgG MoAb
Does not cross-react with sAPP-beta-w or full-length APP-sw/APP-w
sAPP-alpha
(C-terminus)
11088A, BWB
IP
Human(2B3)
Mouse IgG MoAb
Does not cross-react with sAPP-beta or full-length APP

Cross-reacts with mouse/rat sAPP-alpha
sAPP-beta-w
(C-terminus)
18957A, BWB
IP
Human(n/a)
Rabbit IgG
Does not cross-react with sAPP-alpha or full-length APP

 

Additional Information

Please see a product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price Units Select
27731A Human sAPP, Total (highly sensitive) Assay Kit - IBL 96-Well $606.00
27732A Human sAPP beta-w (highly sensitive) Assay Kit - IBL 96-Well $606.00
27734A Human sAPP alpha (highly sensitive) Assay Kit - IBL 96-Well $606.00
10321A Anti-Human sAPP beta-sw (6A1) Mouse IgG MoAb 10 ug $113.00
10321B Anti-Human sAPP beta-sw (6A1) Mouse IgG MoAb 100 ug $499.00
11088A Anti-Human sAPP alpha(2B3) Mouse IgG MoAb 10 ug $113.00
11088B Anti-Human sAPP alpha(2B3) Mouse IgG MoAb 100 ug $499.00
18957B Anti-Human sAPP beta-Wild Type Rabbit IgG Affinity Purify 100 ug $499.00
18957A Anti-Human sAPP beta-Wild Type Rabbit IgG Affinity Purify 10 ug $113.00
27419A Mouse/Rat sAPPa (highly sensitive) Assay Kit - IBL 96-Well $930.00
 

 

 
 

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