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Cell Biology & Epigenetics


Products >  Cell_Biology_and_Epigenetics >  Alzheimers_Disease >  Amyloid_Precursor_Protein

Amyloidogenic Pathway Protein Detection

The accumulation of amyloid-beta protein in the cerebral cortex in Alzheimer's disease (AD) is one of the outcomes of the aberrant proteolytic cleavage of amyloid precursor protein (APP). Aberrant cleavage can occur due to the more than 50 mutations documented in the APP gene that are associated with early-onset Alzheimer's disease.

In the amyloidogenic pathway, cleavage of APP by beta-site APP cleaving enzyme 1 (BACE1), also known as beta-secretase, results in a beta-C-terminal fragment (beta-CTF). This fragment is further cleaved by gamma-secretase to produce insoluble amyloid beta and soluble APP-beta proteins. Amyloid-beta proteins that build up in brain parenchyma are mostly produced from APP expressed in neurons, APP695. A different form of APP, APP770, has been detected in cerebrovascular endothelial cells. Amyloid beta derived from APP770 can accumulate on cerebrovascular walls.

BACE1 is a transmembrane protein that contains two aspartate residues in its active site. Elevated levels of BACE1 have been correlated with late-onset sporadic AD.

ELISAs for APP beta-CTF and APP770 detection

These kits are solid-phase sandwich ELISAs using two antibodies that are highly specific to human APP770 or APP beta-CTF protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This kit can be used to measure human APP770 or APP beta-CTF protein in EDTA plasma, cerebrospinal fluid, cell culture cupernatant, or cell lysates.

Antibodies for APP and BACE1 Detection

The Anti-APP antibody products (Cat. # 11090A, B; 18961A, B; 28021A, B; 28053A, B; 28055A, B; and 28133A, B) consist of affinity-purified IgG antibodies that recognize variants of APP. These antibodies are raised in rabbit or mouse using synthetic peptides and can be applied to Western blot (WB) detection, immunoprecipitation (IP), and immunohistochemical (IHC) detection (Cat. # 28133A, B only) of the indicated APP protein variants.

The Human BACE1 Antibody products are affinity-purified IgG antibodies that recognize human BACE1 protein. The BACE1 antibodies are raised in rabbit using synthetic peptides and can be applied to Western blot (WB) and immunohistochemistry (IHC) detection as well as immunoprecipitation (IP) of human BACE1 protein.

  At-A-Glance   Documents   Images & Data

Features

Antigen/Peptide
Cat. #
Application
Species
(Clone) and Source
Cross‑Reactivity
APP beta-CTF27726AELISAHumanCapture:
(n/a)
Rabbit IgG 

Detect: HRP (82E1)
Mouse IgG MoAb
Does not cross‑react with human sAPP-alpha, sAPP-beta, or sAPP-beta-sw
APP770 27736A ELISAHumanCapture:
(351)
Rabbit IgG 

Detect: HRP (R101A4)
Mouse IgG MoAb
Does not cross-react with human APP695 or APP751
APP77028133A, BWB
IHC
Human(351)
Rabbit IgG
n/a
APP
(N-terminus)
11090A, BWB
IP
Human(10D1)
Mouse IgG MoAb
Does not cross-react with mouse APP

Reacts with all isoforms of APP695, APP751, and APP770, and soluble APP (sAPP-alpha and -beta)
APP
(C-terminus)
18961A, BWB
IP
Human(n/a)
Rabbit IgG
Cross-reacts with mouse and rat APP

Reacts with all isoforms: APP695, APP751, and APP770

Does not react with soluble APP (sAPP-alpha or -beta)
APP (phosphorylated)28021A, BWB
IP
Human(n/a)
Rabbit IgG
Cross-reacts with mouse phosphorylated APP

Does not cross-react with non-phosphorylated APP
APP 28053A, BWB
Human(18)
Rabbit IgG
Cross-reacts with mouse and rat APP

Cross-reacts with sAPP-alpha and sAPP-beta
sAPP-alpha
(C-terminus)
28055A, BWB
IP
Mouse Rat(597)
Rabbit IgG
Does not cross-react with human sAPP-alpha, or human/mouse/rat sAPP-beta

 

Additional Information

Please see a product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price Units Select
11090A Anti-Human APP (N) (10D1) Mouse IgG MoAb 10 ug $113.00
11090B Anti-Human APP (N) (10D1) Mouse IgG MoAb 100 ug $499.00
18961A Anti-Human APP (C) Rabbit IgG Affinity Purify 10 ug $113.00
18961B Anti-Human APP (C) Rabbit IgG Affinity Purify 100 ug $499.00
28053A Anti-APP (18) Rabbit IgG Affinity Purify 10 ug $113.00
28053B Anti-APP (18) Rabbit IgG Affinity Purify 100 ug $499.00
28055A Anti-Mouse/Rat APP (597) Rabbit IgG Affinity Purify 10 ug $113.00
28021A Anti-APP (Phosphorylated) Rabbit IgG Affinity Purify 10 ug $113.00
28021B Anti-APP (Phosphorylated) Rabbit IgG Affinity Purify 100 ug $499.00
28055B Anti-Mouse/Rat APP (597) Rabbit IgG Affinity Purify 100 ug $499.00
27776A Human APP beta CTF Assay Kit - IBL 96-Well $606.00
27736A Human APP770 Assay Kit - IBL 96-Well $849.00
28133A Anti-Human APP770 (351) Rabbit IgG Affinity Purify 10 ug $114.00
28133B Anti-Human APP770 (351) Rabbit IgG Affinity Purify 100 ug $525.00
 

 

 
 

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