The accumulation of amyloid-beta protein in the cerebral cortex in Alzheimer's disease (AD) is one of the outcomes of the aberrant proteolytic cleavage of amyloid precursor protein (APP). Aberrant cleavage can occur due to the more than 50 mutations documented in the APP gene that are associated with early-onset Alzheimer's disease.
In the amyloidogenic pathway, cleavage of APP by beta-site APP cleaving enzyme 1 (BACE1), also known as beta-secretase, results in a beta-C-terminal fragment (beta-CTF). This fragment is further cleaved by gamma-secretase to produce insoluble amyloid beta and soluble APP-beta proteins. Amyloid-beta proteins that build up in brain parenchyma are mostly produced from APP expressed in neurons, APP695. A different form of APP, APP770, has been detected in cerebrovascular endothelial cells. Amyloid beta derived from APP770 can accumulate on cerebrovascular walls.
BACE1 is a transmembrane protein that contains two aspartate residues in its active site. Elevated levels of BACE1 have been correlated with late-onset sporadic AD.
ELISAs for APP beta-CTF and APP770 detection
These kits are solid-phase sandwich ELISAs using two antibodies that are highly specific to human APP770 or APP beta-CTF protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This kit can be used to measure human APP770 or APP beta-CTF protein in EDTA plasma, cerebrospinal fluid, cell culture cupernatant, or cell lysates.
Antibodies for APP and BACE1 Detection
The Anti-APP antibody products (Cat. #11090A, B; 18961A, B; 28021A, B; 28053A, B; and 28055A, B) consist of affinity-purified IgG antibodies that recognize variants of APP. These antibodies are raised in rabbit or mouse using synthetic peptides and can be applied to Western blot (WB) detection or immunoprecipitation (IP) of the indicated APP protein variants.
The Human BACE1 Antibody products are affinity-purified IgG antibodies that recognize human BACE1 protein. The BACE1 antibodies are raised in rabbit using synthetic peptides and can be applied to Western blot (WB) and immunohistochemistry (IHC) detection as well as immunoprecipitation (IP) of human BACE1 protein.