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Cell Biology & Epigenetics


Products >  Cell_Biology_and_Epigenetics >  Alzheimers_Disease >  Amyloid_Beta

Human Amyloid-Beta Detection

The accumulation of amyloid-beta protein plaques in the cerebral cortex is a hallmark of Alzheimer's disease (AD). Amyloid-beta is produced by the proteolytic cleavage of amyloid precursor protein (APP), which is mediated by the enzymes beta- and gamma-secretase. This cleavage reaction produces a number of peptides ranging from 36 to 43 amino acids in length. Our antibodies and ELISA kits detect a variety of human amyloid-beta variants and oligomers.

ELISAs for Human Amyloid-Beta Detection

Amyloid-beta insoluble protein is the main component of plaques found in the brains of patients with AD; however, recent research suggests that soluble, oligomeric forms of Amyloid-Beta are also involved in this disease. The Amyloid-beta Oligomers ELISA (Cat. #27725A) and the Human Amyloid-beta ELISA (Cat. #27729A) are solid-phase sandwich ELISAs that use two highly specific antibodies—one is precoated onto the ELISA plate and the other is HRP-conjugated. The Amyloid-beta Oligomers ELISA kit detects human amyloid-beta oligomers containing two or more epitopes in the N-terminus that are recognized by the anti-human amyloid-beta (82E1) mouse monoclonal antibody. The Human Amyloid-beta ELISA can detect all amyloid-beta variants that are cleaved at the C-terminal side; however, it cannot detect variants fewer than 16 amino acids in length. Both kits can be used to detect amyloid-beta in EDTA-plasma, cerebrospinal fluid, serum, cell culture media, or brain extract.

Antibodies for Human Amyloid-Beta Detection

These products are affinity-purified IgG antibodies that recognize human amyloid-beta variants cleaved at the N-terminus or C-terminus. Our human amyloid-beta antibodies are raised in mice (Human Amyloid-beta Antibodies, Cat. #10027, 10323, 10326 & 10379) and in rabbits (Anti-Human Amyloid-beta Rabbit IgG Antibody, Cat. #18584) using a synthetic peptide. These antibodies are suitable for Western blot (WB) detection, immunohistochemical (IHC) detection, and immunoprecipitation (IP).

  At-A-Glance   Documents   Images & Data

Features

Antigen/Peptide
Cat. #
Application
Species
(Clone) and Source
Cross‑Reactivity
Amyloid-beta oligomer (N-terminus; 1-16 dimer)27725AELISAHumanCapture:
(82E1)
Mouse IgG MoAb

Detect: HRP (82E1)
Mouse IgG MoAb
0.27% cross‑reactivity with amyloid-beta (1-40) peptide

Does not cross-react with mouse or rat amyloid-beta oligomers
Amyloid-beta (1-x) variants cleaved at C-terminus127729AELISAHumanCapture:
(82E1)
Mouse IgG MoAb

Detect: HRP (11-28)
Mouse IgG MoAb
Does not cross‑react with mouse or rat amyloid-beta variants

≤0.1% cross-reactivity against N-terminal deletions or C-terminal deletions fewer than 16 amino acids
Amyloid-beta (11-28)10027A, BIHC
WB
IP
Human(12B2)
Mouse IgG MoAb
Cross-reacts with human amyloid-beta (1-40), (1-42), and (1-43)
Amyloid-beta (1-16)10323A, B
10326A, B2
IHC
WB
IP
Human(82E1)
Mouse IgG MoAb
Does not cross-react with mouse or rat amyloid-beta (1-16)
E22P-Amyloid-beta (10-35)10379A, BIHC
WB
IP
Human(11A1)
Mouse IgG MoAb
Cross-reacts with native human amyloid-beta (1-40), (1-42)
Amyloid-beta (N-terminus)18584A, B, CIHC
WB
IP
Human(n/a)
Rabbit IgG
Cross‑reacts with human amyloid-beta (1-40), (1-42), and (1-43)

1 Cannot detect variants smaller than amyloid-beta (1-16).
2 Cat. #10326A, B are conjugated to biotin.

 

Additional Information

Please see a product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price Units Select
10027A Anti-Human Amyloid beta (11-28) (12B2) Mouse IgG MoAb 5 ug $113.00
10027B Anti-Human Amyloid beta (11-28) (12B2) Mouse IgG MoAb 50 ug $499.00
10323A Anti-Human Amyloid beta (N) (82E1) Mouse IgG MoAb 5 ug $113.00
10323B Anti-Human Amyloid beta (N) (82E1) Mouse IgG MoAb 50 ug $499.00
10326A Anti-Human Amyloid beta (N) (82E1) Mouse IgG MoAb Biotin 5 ug $113.00
10326B Anti-Human Amyloid beta (N) (82E1) Mouse IgG MoAb Biotin 50 ug $499.00
10379A Anti-Human Amyloid beta E22P (11A1) Mouse IgG MoAb 5 ug $113.00
10379B Anti-Human Amyloid beta E22P (11A1) Mouse IgG MoAb 50 ug $499.00
18584A Anti-Human Amyloid beta (N) Rabbit IgG Affinity Purify 10 ug $113.00
18584B Anti-Human Amyloid beta (N) Rabbit IgG Affinity Purify 2 x 5 ug $113.00
18584C Anti-Human Amyloid beta (N) Rabbit IgG Affinity Purify 100 ug $499.00
27725A Human Amyloid beta Oligomers (82E1-specific) Assay Kit - IBL 96-Well $905.00
27729A Human Amyloid beta (1-x) Assay Kit - IBL 96-Well $606.00
 

 

 
 

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