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Cell Biology & Epigenetics


Products >  Cell_Biology_and_Epigenetics >  Alzheimers_Disease >  Alpha2_6-Sialyltransferase

Alpha-2,6-Sialyltransferase Detection

The accumulation of amyloid-beta plaques in the cerebral cortex during Alzheimer's disease (AD) is only one outcome of the proteolytic cleavage of amyloid precursor protein (APP). Over 50 different mutations in the APP gene increase the chances of amyloid-beta plaque generation, leading to early-onset AD. Cleavage of mutated APP by beta-secretase (BACE1) then gamma-secretase—the amyloidogenic pathway—leads to production of pathologic, insoluble amyloid-beta deposits as well as non-pathologic, soluble APP-beta (sAPP-beta), while cleavage by alpha-secretase—the nonamyloidogenic pathway—results in only non-pathogenic sAPP-alpha.

Alpha2 6-sialyltransferase (ST6Gal I) is a glycosyltransferase involved in the biosynthesis of sugar chains. ST6Gal I catalyzes the transfer of an alpha-2,6-linked sialic acid to the N-glycans of certain receptors. Studies have shown that ST6Gal I is cleaved by BACE1, both in vitro and in vivo, to produce a peptide known as ST6Gal I (E41 Form). In humans, cleavage of ST6Gal I by BACE1 generates alpha-2,6-sialyltransferase (E44 form), while in rats it generates alpha-2,6-sialyltransferase (E41 form).

Alternative names: alpha-2,6-ST1, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1, sialyltransferase 1 (beta-galactoside alpha-2,6-sialytransferase), beta-galactoside alpha-2,6-sialyltransferase 1, sialyltransferase 1, 1ST6N, and SIAT1.

ELISAs for Alpha-2,6-Sialyltransferase Detection

These kits are solid-phase sandwich ELISAs using antibodies that are highly specific to human, mouse, and rat ST6Gal I (Cat. # 27762A) or the E41 form in rat (Cat. # 27408A). These ELISAs can be used to measure ST6Gal I in serum, EDTA plasma, or cell culture supernatant.

Antibodies for Alpha-2,6-Sialyltransferase Detection

The ST6Gal I antibody products are an affinity-purified antibodies raised in rabbit using synthetic peptides that recognize ST6Gal I protein. These antibodies and can be used for Western blot (WB) detection of ST6Gal I protein.

  At-A-Glance   Documents   Images & Data
Antigen
Cat. #
Application
Species
(Clone) and Source
Cross‑Reactivity
ST6Gal I27762AELISAHuman Rat MouseCapture:
(STC)
Rabbit IgG

Detect: HRP
(M-2)
Rabbit IgG
n/a
ST6Gal I (E41 form)27408AELISARatCapture:
(n/a)
Rabbit IgG

Detect: HRP
(n/a)
Rabbit IgG
n/a
ST6Gal I (E41 form)18983A, BWBRat(n/a)
Rabbit IgG
Does not cross‑react with human or mouse ST6Gal I
ST6Gal I (C‑terminus)18985A, BWBHuman Rat Mouse(n/a)
Rabbit IgG
Reacts with human, mouse and rat ST6Gal I
ST6Gal I (C‑terminus)28047A, BWBHuman Rat Mouse(n/a)
Rabbit IgG
Reacts with human, mouse and rat ST6Gal I (cleaved and full-length)

Additional Information

Please see the product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price Units Select
18983A Anti-Rat alpha2, 6-Sialyltransferase (E41) Rabbit IgG Affinity Purify 10 ug $113.00
18983B Anti-Rat alpha2, 6-Sialyltransferase (E41) Rabbit IgG Affinity Purify 100 ug $499.00
18985A Anti-alpha2, 6-Sialyltransferase (C) Rabbit IgG Affinity Purify 10 ug $113.00
18985B Anti-alpha2, 6-Sialyltransferase (C) Rabbit IgG Affinity Purify 100 ug $499.00
28047A Anti-alpha2, 6-Sialyltransferase (M2) Rabbit IgG Affinity Purify 10 ug $113.00
28047B Anti-alpha2, 6-Sialyltransferase (M2) Rabbit IgG Affinity Purify 100 ug $499.00
27762A alpha2, 6-Sialyltransferase Assay Kit - IBL 96-Well $606.00
27408A Rat alpha2, 6-Sialyltransferase (E41 Form) Assay Kit - IBL 96-Well $606.00
 

 

 
 

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