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That's Good Science!â„¢ Video | RNA-Seq
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3

RNA-Seq Shows its Sensitive Side

(runtime: 2:17 min)

Next generation sequencing has led to diverse RNA-Seq methods, but one drawback is sensitivity. Conventional RNA-Seq requires >10 ng of RNA from 50–100 cells. But increasingly researchers are interested in profiling gene expression in single cells. Ramskold and colleagues used the SMARTer® Ultra Low Input RNA Kit for Illumina® sequencing to investigate gene expression in a single circulating tumor cell, starting with a mere 10 pg of RNA. — That's Good Science!

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