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Products >  cDNA_Synthesis_and_Library_Construction >  Next_Gen_Sequencing_Kits >  Transcriptome_Analysis_Overview

SMARTer cDNA Synthesis Kits for Next-Gen Sequencing

SMARTer cDNA synthesis is the industry standard for experiments that require exceptional sensitivity and complete transcriptome coverage, because it synthesizes accurate, full-length cDNA directly from cells or total RNA. The same core technology is included in a variety of SMARTer kits, designed for use with sorted cells, LCM or FFPE samples, and total RNA. Kits are available for leading sequencing platforms and Fluidigm’s C1 system.

 

SMARTer Selection Guide

APPLICATION
Transcriptome Analysis
mRNA-Seq
RNA-Seq
with Strand Information
RNA-Seq

(dT priming)

(N6 priming)*

(N6 priming)*

 

* Ribosomal RNA removal required; use w/ RiboGone - Mammalian

SAMPLE TYPE
     
a single cell or a few cells
(intact RNA; RIN 7–10)
1
2
6
LCM samples, FFPE tissue,
prokaryotic samples,
or samples with degraded RNA
(low-quality RNA; RIN 2–7)
3+RG
4+RG
5+RG
eukaryotic total RNA
(> 100 ng)
3+RG
4+RG
5+RG
1 SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing
2 SMARTer Ultra Low RNA Kit for Illumina Sequencing-HV
3 SMARTer Stranded RNA-Seq Kit
4 SMARTer Universal Low Input RNA Kit for Sequencing
5 SMARTer Universal Low Input RNA Library Prep Kit
6 SMARTer Ultra Low RNA Kit for the Fluidigm C1 System
RG RiboGone™ - Mammalian (for Ribosomal RNA Removal)
INSTRUMENT COMPATIBILITY
Illumina platform
Fluidigm C1 cell capture platform
Compatible with Illumina
and Ion Torrent platforms

 

4 RG 5 RG 4 RG 5 RG 3 RG 3 RG"
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