SMARTer Stranded RNA-Seq Kits—Strand-Specific Library Construction for Transcriptome Analysis on Illumina Platforms
The SMARTer Stranded RNA-Seq Kit provides a solution for generating libraries for Illumina® sequencing platforms that retain strand information with >99% accuracy. This kit generates robust data with input as low as 100 pg to 100 ng of input RNA. it is recommended to use ribosomal RNA depleted or poly A purified RNA as input. The SMARTer Stranded RNA-Seq Kit uses a random priming method that is applicable to both polyA RNA and rRNA-removed total RNA. The kit can be used with both mammalian RNA and bacterial samples. The core SMART technology has been modified in this kit to generate complete transcriptome coverage of both coding and non-coding RNA while incorporating indexes and adapters in the workflow, which eliminates the need for a downstream library preparation kit. You can generate indexed libraries that are ready for Illumina sequencing in less than 4 hours using the SMARTer Stranded RNA-Seq Kit protocol. We also offer the SMARTer Stranded RNA-Seq Kit HT includes 8 Forward Primers and 12 Reverse Primers to generate up to 96 separate indexes for high-throughput reactions.
For rRNA removal from 10–100 ng of input total RNA, we recommend the RiboGone - Mammalian kit. The SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian combines SMARTer Stranded technology with RiboGone rRNA removal for complete, seamless RNA-seq library preparation from total RNA samples in <5 hours.