SMARTer Stranded RNA-Seq Kits—Strand-Specific Library Construction for Transcriptome Analysis on Illumina Platforms
The SMARTer Stranded RNA-Seq Kit provides a solution for generating libraries for Illumina® sequencing platforms that retain strand information with >99% accuracy, and yields robust data from as little as 100 pg to 100 ng of input RNA. The SMARTer Stranded RNA-Seq Kit uses a random priming method that is compatible with eukaryotic or prokaryotic RNA, and is recommended for use with rRNA-depleted or poly(A)-enriched samples. The core SMART technology has been modified in this kit to generate complete transcriptome coverage of both coding and non-coding RNA while incorporating indexes and adapters in the workflow, which eliminates the need for a downstream library preparation kit. Indexed libraries that are ready for Illumina sequencing can be prepared in less than 4 hours using the SMARTer Stranded RNA-Seq Kit protocol. We also offer the SMARTer Stranded RNA-Seq Kit HT, which includes 8 Forward Primers and 12 Reverse Primers to generate up to 96 separate indexes for high-throughput applications.
For rRNA removal from 10–100 ng of input total RNA, we recommend the RiboGone - Mammalian kit. The SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian combines SMARTer Stranded technology with RiboGone rRNA removal for complete, seamless RNA-seq library preparation from total RNA samples in <5 hours.