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cDNA Synthesis & Library Construction


Products >  cDNA_Synthesis_and_Library_Construction >  Next_Gen_Sequencing_Kits >  Single_cell_RNA_Seq_Kits_for_mRNA_seq >  Single_Cell_RNA_Seq_dT_Primed_Fluidigm_C1

SMART-Seq v4 Ultra Low RNA Kit for the Fluidigm C1 System—High Performance Single-Cell Transcriptome Analysis

Single-cell transcriptomics is important in understanding how gene expression of individual cells varies within cell populations. Takara Clontech has developed robust tools compatible with single cells isolated in Fluidigm’s integrated fluidic circuit (IFC) system. The sensitive SMARTer chemistry of our ultra-low input kits, paired with the high-throughput automation capability of the Fluidigm C1 system, allow high-resolution insight into transcriptomics.

We offer two generations of single-cell mRNA-seq kits for the Fluidigm C1 Single Cell Auto Prep system. The newest version, the SMART-Seq v4 Ultra Low Input RNA Kit for the Fluidigm C1 System, incorporates features from the industry-leading SMART-Seq v4 kit, including SMART (Switching Mechanism at 5’ End of RNA Template) and locked nucleic acid (LNA) technology, which are collectively referred to as "SMARTer-seq® chemistry".

This kit outperforms previously-published protocols and the earlier generation; data from the kit shows high sensitivity, better reproducibility, and an improved representation of GC- and AT-rich genes. The new script for using this kit is supported by the mRNA Seq IFC and the Open App IFC, and can be downloaded through the Fluidigm Script Hub.

  At-A-Glance   Documents   Resources

Features

  • Improved SMART-technology-based chemistry—SMART-Seq v4 oligo with LNA technology plus improved formulations lead to better performance (high sensitivity, better reproducibility, and more GC-rich genes detected)
  • Library construction in 2 days—cDNA synthesis protocol takes approximately two hours, followed by the eight-hour SMART-Seq v4 script on the C1 instrument. The entire library construction protocol can be completed within two working days.
  • New script—supported for use on both the mRNA Seq IFC and the Open App IFC. The SMART-Seq v4 script is available at the Fluidigm Script Hub. The user manual for this product is available on the Documents tab of this web page.

Interested in more data and FAQs about this product? Visit the NGS Resource Portal.

Applications

  • cDNA synthesis for mRNA-seq from up to 96 single cells on the Fluidigm C1 System
  • cDNA synthesis for qPCR analysis on the Fluidigm Biomark platform prior to Illumina sequencing

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
New Cat. # Product Contents Size Price License Units Select
635025 SMART-Seq® v4 Ultra® Low Input RNA Kit for the Fluidigm® C1™ System, 4 IFCs Each $1,344.00 License Statements
635026 SMART-Seq® v4 Ultra® Low Input RNA Kit for the Fluidigm® C1™ System, 10 IFCs Each $2,688.00 License Statements
  634832 SMARTer® Ultra® Low RNA Kit for the Fluidigm® C1™ System, 2 IFCs Each $1,050.00 License Statements
  634833 SMARTer® Ultra® Low RNA Kit for the Fluidigm® C1™ System, 10 IFCs Each $3,800.00 License Statements
 

 

 
 

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