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Viral Transduction


Products >  Viral_Transduction >  Retroviral_Transduction_Tools >  amphotropic_ecotropic_receptor_boosters

Ecotropic and Amphotropic Receptor Boosters

Ecotropic and Amphotropic Receptor Booster technologies are used to temporarily elevate the density of viral receptor proteins on the surface of any target cell that you wish to infect. The technology allows you to efficiently transduce cells that are resistant to viral infection due to low receptor density. The Ecotropic Receptor Booster, for example, allows you to efficiently transduce human cells with ecotropic retrovirus or lentivirus that otherwise can only infect rodent cells.

How Does it Work?

Each receptor booster consists of a vial of concentrated exosome-like vesicles that are coated with either the mCAT-1 receptor protein (ecotropic) or hPit2 receptor protein (amphotropic). When the booster vesicles are applied to your cells (10–20 µl per well of a 6-well plate for ~2 hr) they fuse with the plasma membrane, resulting in an increased level of the receptor on the cell surface. Your target cells can then be more efficiently transduced with ecotropic or amphotropic retrovirus.

We Recommend the Following Products for Producing Virus:

At-A-Glance Documents Images & Data

Features

  • Increase efficiency of transduction by temporarily increasing the concentration of viral receptor proteins on your target cells
  • Simply pre-incubate your target cells with these concentrated exosome-like vesicles for 2 hr prior to infection with virus
  • Infect human cells with ecotropic retrovirus or ecotropic lentivirus
  • The Ecotropic Receptor Booster (Cat. No. 631471) delivers the mCAT-1 receptor to target cells
  • The Amphotropic Receptor Booster (Cat. No. 631469) increases the surface density of the hPit2 receptor on target cells so that they can be more efficiently transduced with amphotropic retrovirus

Applications

  • Increased transduction efficiency of hard-to-infect cell types
  • Transduce human cells with ecotropic retrovirus or lentivirus

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
New Cat. # Product Package Size Price License # of Units Select
631469 Amphotropic Receptor Booster 20 Rxns $245.00 License Statements
  631471 Ecotropic Receptor Booster 20 Rxns $245.00 License Statements
 

 

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