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Viral Transduction


Products >  Viral_Transduction >  Lentiviral_Transduction_Tools >  Lentivirus_Titration >  Lentivirus_Titration_Overview

Lentiviral Titration Overview

Lenti-X GoStix Instant Titration

  • Get a YES or NO in only 10 minutes
  • Distinguish between good and bad preps
  • Know when to harvest lentiviral supernatants

Apply 20 µl of your lentiviral supernatant to the sample well, add 4 drops of buffer, and wait for the indicator bands to appear. Two bands mean you have > 5 × 105 IFU/ml.

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Lenti-X p24 Rapid Titer Kit

  • Fast and easy ELISA-based titration protocol
  • Collect supernatant, lyse, bind, wash, and detect
  • Correlate p24 capsid protein content to virus

Lentiviral supernatant is bound to anti-p24 coated wells and detected using a combination of biotinylated anti-p24 secondary antibody, streptavidin-HRP, and a development reagent.

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Lenti-X qRT-PCR Titration Kit

  • Fast and accurate titration using SYBR Green chemistry
  • Harvest, titer, and infect in one day
  • Obtain results in 4 hr

Set up the qRT-PCR reaction with purified RNA from harvested lentiviral supernatant. Determine the viral genome content from a calibrated RNA standard curve.

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Lenti-X Provirus Quantification Kit

  • Rapidly measure copy number of integrated lentivirus (provirus)
  • Determine accurate effective lentivirus titer
  • Compare transduction data across multiple cell lines

Quantify the number of lentiviruses that have integrated into the nuclear DNA of your target cells using a SYBR Green-based qPCR reaction.

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