The Lenti-X HTX Ecotropic Packaging System produces high-titer lentivirus pseudotyped with the mCAT-1 envelope glycoprotein, which allows you to limit transduction to mouse and rat cells. It is also possible to transduce human cell types with ecoptropic lentivirus—but only if you pre-treat those cells with Ecotropic Receptor Booster, which allows for a high infection efficiency regardless of the cell line used.
Lenti-X HTX Ecotropic Packaging System
The Lenti-X HTX Ecotropic Packaging System produces lentivirus pseudotyped with the MLV ecotropic envelope glycoprotein, which allows you to limit transduction to mouse and rat cells. The system uses a 4th generation lentiviral packaging technology resulting in high expression of the viral components when transfected into the Lenti-X 293T Cell Line (sold separately). The kit contains the following components:
- Lenti-X HTX Ecotropic Packaging Mix 2
- Xfect Transfection Reagent (Cat. No. 631317)
- Tet System Approved FBS, US Sourced (Cat. No. 631105)
- Lenti-X GoStix (Sample)
Ecotropic Receptor Booster
The Ecotropic Receptor Booster is used to temporarily elevate the density of the ecotropic receptor protein mCAT-1 on the surface of any target cell that you wish to infect. For example, this technology can be used to efficiently transduce human cells with ecotropic retrovirus or lentivirus that otherwise could only infect rodent cells. The technology also allows you to increase the infection efficiency of any type of cells that are resistant to viral infection, generating a high transfection efficiency regardless of the cell type used.