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Products >  Viral_Transduction >  AAV_Transduction_Tools >  qPCR_Titration_Kit

Determining AAV Titer by Real-Time PCR: AAVpro Titration Kit 

The AAVpro Titration Kit (for Real Time PCR) Ver. 2 contains all of the reagents necessary for extraction of adeno-associated virus (AAV) particles from AAV-producing cells and real-time PCR analysis of a viral genomic sequence. With this kit, amplification of the ITRs (inverted terminal repeats) of AAV2 is used to estimate titer. This kit allows for more precise and rapid quantification of viral titer than conventional DNA blot and ELISA methods.

Titration of any AAV Serotype

The serotype of AAV is determined by the type of Cap gene. Regardless of the serotype, the viral genome encapsulated within recombinant AAV particles typically contains the ITRs of AAV2 (commonly used constructs). This kit can be used for determining the titer of any AAV serotype, as long as the ITRs are derived from AAV2.

At-A-Glance Documents Images & Data Resources

Features

  • Can be used to determine the titer of AAV of any serotype as long as the ITRs are from AAV2 (most commonly used contructs)
  • Kit contains all reagents necessary for extraction of AAV particles from virus-producing cells and real-time PCR assay of AAV2 ITRs
  • Simple and fast titration method—results in approximately 2.5 hours
  • High sensitivity titration—more precise quantification than conventional DNA blot and ELISA methods

Applications

  • Determining the titer of AAV using real-time PCR
  • Rapid and precise titration of any AAV serotype; quantification is based on the ITRs of AAV2, a sequence that present in commonly used AAV vectors

Additional Product Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the User Manual for detailed protocols. The Certificate of Analysis and User Manual are located under the Documents tab.


 
 
Products
New Cat. # Product Package Size Price License # of Units Select
6233 AAVpro™ Titration Kit (for Real Time PCR) Ver.2 100 Rxns $422.00 License Statements
 

 

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