Determining AAV Titer by Real-Time PCR: AAVpro Titration Kit
The AAVpro Titration Kit (for Real Time PCR) Ver. 2 contains all of the reagents necessary for extraction of adeno-associated virus (AAV) particles from AAV-producing cells and real-time PCR analysis of a viral genomic sequence. With this kit, amplification of the ITRs (inverted terminal repeats) of AAV2 is used to estimate titer. This kit allows for more precise and rapid quantification of viral titer than conventional DNA blot and ELISA methods.
Titration of any AAV Serotype
The serotype of AAV is determined by the type of Cap gene. Regardless of the serotype, the viral genome encapsulated within recombinant AAV particles typically contains the ITRs of AAV2 (commonly used constructs). This kit can be used for determining the titer of any AAV serotype, as long as the ITRs are derived from AAV2.