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Viral Transduction


Products >  Viral_Transduction >  AAV_Transduction_Tools >  Extraction_Buffer

Improved Method for AAV Particle Extraction from Virus-Producing Cells: AAVpro Extraction Solution

Increase AAV yield by at least 3-fold with AAVpro Extraction Solution, a reagent for the extraction of AAV particles from AAV particle-producing cells. Isolation of AAV particles is typically performed using freeze-thaw or sonication methods. However, these methods are time consuming and require special equipment. The AAVpro Extraction Solution provides an improved method for AAV particle isolation; simply add the Extraction Solutions to virus-producing cells and recover viral particles by centrifugation. In addition to a simple protocol, the Extraction Solution reduces host protein and nucleic acid contamination, yielding viral particle solutions that are ready for cell infection or further purification with the AAVpro Purification Kit*.
* For in vivo transduction, purify AAV2 particles with the AAVpro Purification Kit (AAV2), which includes this product, to obtain highly-purified particles that do not contain any residual virus-producing cells or impurities from the culture medium.

Note: This product is included in the AAVpro Helper Free System and AAVpro Purification Kit.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • Increased viral particle recovery as compared to conventional freeze-thaw
    methods—expect at least a 3-fold increase in viral yield
  • Simple method for AAV particle extraction from virus-producing cells
  • Resulting viral particle solution contains low amounts of host DNA and protein contamination
  • Can be used for any AAV serotype
  • Extracted AAV particles can be used directly for cell infection or be further purified with the AAVpro Purification Kit

Applications

  • AAV particle extraction from virus-producing cells

Additional Product Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the User Manual for a detailed protocol. The Certificate of Analysis and User Manual are located under the Documents tab.


 
 
Products
New Cat. # Product Contents Size Price License Units Select
6235 AAVpro® Extraction Solution 1 Set $127.00 License Statements
 

 

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