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Transfection & Cell Culture

Products >  Transfection_and_Cell_Culture >  Plasmid_Transfection >  Xfect_Single_Shots

Xfect Single Shots Transfection Reagent

Xfect Single Shots Transfection Reagent is a lyophilized plasmid transfection reagent that comes pre-packaged in a convenient, One Tube=One Transfection format. Simply add your DNA, vortex the tube, and incubate the reaction for 10 minutes. Your plasmid DNA is now ready to be added to your cells. The single tube format of Xfect Single Shots Transfection Reagent enables one plasmid transfection to be performed per each individual tube and requires just two pipetting steps. All reaction components are included in the tube except for the plasmid DNA. The midi kit is designed for transfecting plasmid into cells grown in a well of a 6-well plate.

The transfection reagent contained within Xfect Single Shots Transfection Reagent is the same as that offered in Xfect Transfection Reagent except that it has been accurately pre-aliquoted into single tubes, then lyophilized for individual use. Xfect Transfection Reagent has been proven to enable highly efficient plasmid transfection of a number of cell types, including primary cells and stem cells, without concomitant cellular toxicity. This is because, unlike other transfection products, Xfect Transfection Reagent contains a biodegradable cationic polymer that binds to DNA, resulting in the formation of nanocomplexes that are quickly and efficiently taken up by the cell. Because the Xfect Transfection Reagent is serum-compatible, cells do not become stressed and apoptotic due to the removal of serum or the addition of serum-free medium.

At-A-Glance Documents Images & Data Resources


  • Watch the video.
  • Simple: One Tube=One Transfection. Just add DNA.
  • Easy: Reaction set-up all in ONE tube, see the picture protocol
  • Efficient: High transfection efficiency in a broad range of cell types.
  • Low cellular toxicity: Xfect forms biodegradable nanoparticles with DNA.
  • Xfect is serum-compatible: Keep your cells in FBS- they like it!
  • Low risk of contamination: Individual tubes can be shared with colleagues.
  • Midi size: For transfecting cells grown in a well of a 6-well plate.


  • Plasmid transfection
  • Primary cell transfection
  • Stem cell transfection
  • Mammalian cell transfection
  • Cell culture transfection

Additional Product Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

New Cat. # Product Package Size Price License # of Units Select
631366 Xfect™ Single Shots (Midi) 96 Rxns $358.00 License Statements


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