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Stem Cell Research

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Human iPS Cell Culture Protocol Overview

The DEF-CS system is a complete culture system that includes basal medium as well as culture additives and a surface coating compound. Cells are cultured in the absence of feeder cells, streamlining passaging. In addition, cells are passaged as single cells, a method that is less complicated than traditional passaging techniques and is ideal for single-cell applications.

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Coat new cell culture plates with DEF-CS COAT-1a
Media preparation
Mix DEF-CS Basal medium with growth factors (DEF-CS GF-1, GF-2 and GF-3)
Use TrypLE Select Enzyme (Life Technologies) to detach the cells
Cell counting
Add media to the single cell suspension and count the cells
Dilute the cells in complete DEF-CS media to achieve a plating density of 4.0–5.0 x 104 cells/cm2
Remove excess coating substrate and plate the cells
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