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Real-Time qPCR & Reverse Transcription

Products >  Real-Time_qPCR_and_Reverse_Transcription >  AMV_RT >  Two-Step_RT-PCR >  High_GC_or_Secondary_Structure


The BcaBEST RNA PCR Kit is designed to perform both reverse transcription and DNA amplification in a single tube. This two step RT-PCR kit takes advantage of the BcaBEST DNA Polymerase, which contains both DNA polymerase and reverse transcriptase properties, to enable first strand cDNA synthesis. Compared with standard reverse transcriptases, BcaBEST polymerase operates at an optimal temperature of 65°C, permitting RT-PCR of long or difficult RNA templates. RT-PCR troubleshooting of highly structured RNA templates is especially recommended using the BcaBEST RNA PCR kit.

Bca-Optimized Taq Polymerase is used for second strand synthesis and subsequent PCR. The Bca-Optimized Taq Polymerase utilizes LA PCR technology for improved PCR amplicon length and accuracy. Random 9-mers, oligo-dT primers or specific downstream primers (that function as antisense PCR primers) can be used for cDNA synthesis.

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  • RT-PCR of Long or Difficult RNA Templates. Especially useful for GC-rich RNA or high secondary structure RNA
  • Amplification of templates up to 5 kb in length
  • Transcription at an optimum reaction temperature of 65°C
  • Uses Bca-Optimized Taq Polymerase, an LA technology-based enzyme that is optimized for use with BcaBEST DNA Polymerase
  • Kit includes random 9-mers or oligo-dT primers for first strand cDNA synthesis. Specific downstream primers can be prepared by the user.
  • Procedure is performed in a single tube
  • Reverse transcription is stopped via enzyme heat inactivation. PCR mixture is added directly to the tube and amplified.


  • Single-tube RT-PCR, using LA PCR technology for better PCR performance
  • Reverse transcription of GC-rich or high secondary structure RNA



BcaBEST DNA Polymerase (22 U/µL)50 µL
RNase Inhibitor (40 U/µL)25 µL
Random 9-mers (50 µM) 50 µL
Oligo dT Primer (50 µM)50 µL
RNase-Free dH2O (DEPC-treated) 1 mL
Bca-Optimized Taq Polymerase (5 U/µL)25 µL
2X Bca 1st Strand Buffer2 x 1.25 mL
5X Bca 2nd Strand Buffer800 µL
dNTP Mixture (10 mM each)50 µL
MgSO4 (25 mM)500 µL
Control F-1 Primer (20 µmol/µL) 25 µL
Control R-1 Primer (20 µmol/µL) 25 µL
Positive Control RNA (1 µg/µL) 25 µL



Cat. # Product Contents Size Price Units Select
RR023A BcaBEST™ RNA PCR Kit Ver. 1.1 100 Rxns $326.00
RR023B BcaBEST™ RNA PCR Kit Ver. 1.1 200 Rxns $557.00


Recommended Products
Cat. # Product Contents Size Price Units Select
2630A AMV Reverse Transcriptase XL for RT-PCR 250 Units $211.00
4030 dNTP Mixture 3.2 µmol Each/1.25 mL $60.00

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