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Protein Interactions & Profiling


Products >  Protein_Interactions_and_Profiling >  Co-immunoprecipitation >  Myc-Tagged_and_HA-Tagged_Vectors

Myc-Tag & HA-Tag Vectors—Mammalian Expression Vectors

These mammalian expression vectors allow you to express a protein of interest fused to either the c-Myc or hemagglutinin (HA) epitope tag. c-Myc and HA are well-characterized and highly immunoreactive tags, and thus are ideal for co-immunoprecipitation studies but are also easily detected via Western blot. Moreover, c-Myc and HA are small tags and thus unlikely to interfere with the function of your protein.

The myc- or HA-tagged protein of interest can be detected by either western analysis or fluorescence microscopy using an anti-myc antibody or anti-HA antibody.

The pCMV-Myc & pCMV-HA Vector Set includes c-Myc Monoclonal and HA-Tag Polyclonal Antibodies, which can also be used in Western blot, ELISA, or immunostaining assays. A sequencing primer is also included in the set to confirm that the insert is in the correct reading frame.

HA Tag sequence ACC ATG TAC CCA TAC GAT GTT CCA GAT TAC GCT

Myc Tag sequence ATG GCA TCA ATG CAG AAG CTG ATC TCA GAG GAG GAC CTG

At-A-Glance Documents Images & Data Resources

Features

  • Co-immunoprecipitation of myc-tagged and HA-tagged proteins in mammalian cells
  • Strong constitutive expression
  • Confirm yeast two-hybrid interactions in mammalian cells
  • Strong clean bands via Western blot

Applications

  • Co-immunoprecipitation studies
  • Confirmation of protein-protein interactions
  • Western blotting
  • Epitope-tagged expression

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Package Size Price # of Units Select
631207 HA-Tag Polyclonal Antibody 100 ug $305.00
631206 c-Myc Monoclonal Antibody 200 ug $313.00
631604 pCMV-Myc & pCMV-HA Vector Set Each $439.00
631992 pEF1alpha-HA Vector 10 ug $402.00
631991 pEF1alpha-Myc Vector 10 ug $402.00
 

 

 


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