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Protein Interactions & Profiling


Products >  Protein_Interactions_and_Profiling >  Co-immunoprecipitation >  Matchmaker_Chemiluminescent_Co-IP_Assay

Chemiluminescent Co-IP Assay for Mammalian Proteins

This co-immunoprecipitation assay is a simple and sensitive tool to quickly confirm that two proteins interact when they are expressed in mammalian cells.

The assay requires the following three components:

  • The Matchmaker Chemiluminescent Co-IP Vector Set enables you construct and strongly express two tagged fusion proteins.
  • The Matchmaker Chemiluminescent Co-IP Kit allows you to co-immunoprecipitate the interacting fusion protein complexes from cell extracts.
  • The ProLabel Detection Kit II (Cat. No. 631629) is used to perform a chemiluminescent assay that directly quantifies precipitated protein complexes—no SDS-PAGE or Western blotting is required.

Co-Immunoprecipitation of Two Proteins

Protein 1 cDNAs are cloned in the pAcGFP1-C vector to construct fusion proteins containing the brightly fluorescent Living Colors AcGFP1 tag. This tag enables fusion protein expression to be visualized under fluorescent microscopy and also functions as the antibody epitope used for immunoprecipitation. Protein 2 cDNAs are cloned in the pProLabel-C vector to incorporate the ProLabel tag, which enables sensitive chemiluminescent detection.

At-A-Glance Documents Images & Data

Features

  • Quickly confirm protein interactions by co-immunoprecipitation in mammalian cells
  • Highly specific assay for protein interactions
  • Visualize expression via fluorescence
  • Quantitative chemiluminescent detection
  • Ideal complement to yeast two-hybrid studies

Applications

  • Co-immunoprecipitation of mammalian proteins
  • Confirmation of yeast two-hybrid interactions

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
Products
Cat. # Product Package Size Price License # of Units Select
630458 Matchmaker® Chemiluminescent Co-IP Vector Set 1 Set $445.00 License Statements *
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 

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