Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Chemiluminescent Co-IP Assay for Mammalian Proteins
Quickly confirm protein interactions by co-immunoprecipitation in mammalian cells
Highly specific assay for protein interactions
Visualize expression via fluorescence
Quantitative chemiluminescent detection
Ideal complement to yeast two-hybrid studies
This co-immunoprecipitation assay is a simple and sensitive tool to quickly confirm that two proteins interact when they are expressed in mammalian cells.
The assay requires the following three components:
The Matchmaker Chemiluminescent Co-IP Vector Set enables you construct and strongly express two tagged fusion proteins.
The Matchmaker Chemiluminescent Co-IP Kit allows you to co-immunoprecipitate the interacting fusion protein complexes from cell extracts.
The ProLabel Detection Kit II (Cat. No. 631629) is used to perform a chemiluminescent assay that directly quantifies precipitated protein complexes—no SDS-PAGE or Western blotting is required.
Co-Immunoprecipitation of Two Proteins
Protein 1 cDNAs are cloned in the pAcGFP1-C vector to construct fusion proteins containing the brightly fluorescent Living Colors AcGFP1 tag. This tag enables fusion protein expression to be visualized under fluorescent microscopy and also functions as the antibody epitope used for immunoprecipitation. Protein 2 cDNAs are cloned in the pProLabel-C vector to incorporate the ProLabel tag, which enables sensitive chemiluminescent detection.