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Protein Expression & Purification


Products >  Protein_Expression_and_Purification >  Reagents >  Capturem_Trypsin

Mini spin columns for rapid, complete protein digestion—Capturem Trypsin

Capturem Trypsin provides fast, efficient, and complete digestion of proteins at room temperature, allowing for an uninterrupted proteomic analysis workflow. This product, based on our novel Capturem technology, provides membrane-immobilized trypsin in a mini spin column format. Capturem Trypsin completely digests protein samples in less than two minutes, compared to the overnight incubations often required for in-solution trypsin, and provides better digestion efficiency with fewer autolyzed trypsin fragments.

More effective proteomic analysis

Protein digestion using Capturem Trypsin is more effective than in-solution trypsin digestion for preparing samples for mass spectrometry (MS) analysis because it eliminates long digestion times which may lead to oxidation and other protein modifications, as well as increased trypsin autolysis, which can make peptide and protein identification more difficult and less reproducible.

Fast, easy protocol

Capturem Trypsin includes Capturem Trypsin mini spin columns and an activation buffer. Capturem Trypsin digestion is performed using a fast, easy protocol. Just load 200 µl of activation buffer, spin for one minute, then load your protein sample and spin for another minute to elute peptides that are ready for downstream analysis.

  At-A-Glance   Documents   Images & Data

Features

  • Digests protein samples in less than two minutes instead of overnight
  • More efficient and complete than in-solution protein digestion
  • Generates fewer autolyzed trypsin fragments and protein modifications
  • Allows an uninterrupted proteomics analysis workflow
  • Enables more effective peptide and protein identification in MS analysis

Applications

  • Protein characterization
  • Proteomic analysis
  • Mass spectrometry sample preparation
  • Antibody characterization

Additional Product Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
New Offers Cat. # Product Contents Size Price License Units Select
635722 Capturem™ Trypsin 20 Rxns $250.00 License Statements
 

 

 
 

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