Combine the advantages of our highly selective TALON chemistry with magnetic bead separation. Magnetic particles in the beads facilitate quick and easy separation of microscale quantities of protein when placed on a magnetic separator. The beads, which are precharged with Co2+, have a higher specificity for his-tagged proteins than nickel-based resins. Co2+ is bound to the beads using TALON’s unique tetradentate metal chelator, which binds cobalt at four sites, virtually eliminating metal leakage during purification.
Highly Specific Binding & Elution
TALON Magnetic Beads bind his-tagged proteins ranging from low to high molecular weight with high specificity. Purified proteins are eluted in small volumes (50–200 µl), resulting in concentrated samples (up to 3 mg/ml). TALON Magnetic Beads are supplied as a 5% suspension with a demonstrated binding capacity of 750 µg of protein per ml of suspension.
Microscale purification with TALON Magnetic Beads can be used to screen expression levels or for protein-protein interaction studies. In addition, the use of TALON chemistry allows for seamless scale-up of purification of target proteins using our standard TALON Resin.