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Protein Expression & Purification


Products >  Protein_Expression_and_Purification >  His-Tagged_Protein_Purification >  Cobalt_Resin-Magnetic

His-Tagged High-Throughput Micro-Scale Purification—TALON Magnetic Beads

Combine the advantages of our highly selective TALON chemistry with magnetic bead separation. Magnetic particles in the beads facilitate quick and easy separation of microscale quantities of protein when placed on a magnetic separator. The beads, which are precharged with Co2+, have a higher specificity for his-tagged proteins than nickel-based resins. Co2+ is bound to the beads using TALON’s unique tetradentate metal chelator, which binds cobalt at four sites, virtually eliminating metal leakage during purification.

Highly Specific Binding & Elution

TALON Magnetic Beads bind his-tagged proteins ranging from low to high molecular weight with high specificity. Purified proteins are eluted in small volumes (50–200 µl), resulting in concentrated samples (up to 3 mg/ml). TALON Magnetic Beads are supplied as a 5% suspension with a demonstrated binding capacity of 750 µg of protein per ml of suspension.

Microscale Screening

Microscale purification with TALON Magnetic Beads can be used to screen expression levels or for protein-protein interaction studies. In addition, the use of TALON chemistry allows for seamless scale-up of purification of target proteins using our standard TALON Resin.

At-A-Glance Documents Images & Data Resources

Features

  • Quick and easy separation of his-tagged proteins
  • Highly selective TALON chemistry for increased purity
  • Eluted samples ideal for small volume applications

Applications

TALON Magnetic Beads provide simple, effective separation of recombinant his-tagged proteins for a wide range of applications, including:

  • Microscale purification of his-tagged proteins
  • Studying protein structure and function
  • Preparing proteins for x-ray crystallography
  • Performing assays that detect protein-protein and protein-DNA interactions
  • Immunization to raise antibodies against a protein of interest
  • Screening for protein expression
  • Optimizing purification conditions for scale-up with TALON Resin

TALON Magnetic Beads can also be used to immobilize 6xHis-tagged proteins for affinity chromatography, in order to:

  • Analyze protein-protein and protein-nucleic acid interactions
  • Purify untagged subunits or nucleic acids that interact with the immobilized protein
  • Perform antibody purification
  • Study interactions between ligands and receptors

Advantages

  • Choice of native or denaturing conditions to purify proteins
  • Directed presentation of 6xHis-tagged proteins increases reproducibility and signal-to-noise ratios
  • Varying the amount of beads per well allows for a wide range of binding capacities
  • A powerful tool for analyzing interactions between biomolecules

 
 
Products
Cat. # Product Package Size Price License # of Units Select
635636 TALON® Magnetic Beads 2 x 1 mL $150.00 License Statements
635637 TALON® Magnetic Beads 6 x 1 mL $432.00 License Statements
635638 TALON® Magnetic Beads Buffer Kit Each $159.00  
 

 

 

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