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Protein Expression & Purification


Products >  Protein_Expression_and_Purification >  His-Tagged_Protein_Purification >  Cobalt_Columns-FPLC

Cobalt Resin FPLC Cartridges for His-Tagged Protein Purification

HisTALON Cartridges are FPLC cartridges prepacked with TALON Superflow Resin, which can withstand flow rates of 5–20 cm per min. This resin combines Superflow 6, a rigid, highly porous agarose derivative, with TALON. The columns prepacked with the resin can be used for efficiently purifying his-tagged proteins from a total soluble protein extract of bacterial, mammalian, or baculovirus-infected cells.

TALON Metal Affinity Resin is a durable immobilized metal affinity chromatography (IMAC) resin that has a remarkable affinity and specificity for his-tagged proteins. TALON Resin is compatible with all commonly used IMAC reagents and allows protein purification under native or denaturing conditions.

TALON Metal Affinity Resin uses cobalt ions for purifying recombinant his-tagged proteins. TALON is a tetradentate chelator charged with cobalt, and is specific for his-tagged proteins. The TALON reactive core, containing cobalt, has strict requirements for the spatial positioning of histidines. Only adjacent histidines or specially positioned, neighboring histidines are able to bind the cobalt in this reactive core. In nickel-based resins (i.e., Ni-NTA Resin), these spatial requirements are less strict. Therefore, nickel-based resins are also able to bind histidines located in places other than the protein’s his-tag.

TALON Superflow is specifically designed for quick and effective purification of his-tagged proteins at high flow rates and medium pressure (up to 150 psi)—it’s ideal for FPLC applications.

At-A-Glance Documents Images & Data Resources

Features

  • Easy-to-use prepacked 1 ml and 5 ml cartridges
  • Maximize yield of biologically active protein
  • Superior purity & high yields

Applications

Purified recombinant his-tagged proteins can be used for:

  • Crystallography
  • Functional assays
  • Structural investigations
  • Other applications

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Package Size Price License # of Units Select
635651 HisTALON™ Buffer Set 20 Purifications $204.00  
635683 HisTALON™ Superflow Cartridge 1 x 5 mL Cartridge $129.00 License Statements
635649 HisTALON™ Superflow Cartridge Purification Kit 5 x 1 mL Cartridges $325.00 License Statements
635681 HisTALON™ Superflow Cartridge Purification Kit 5 x 5 mL Cartridges $693.00 License Statements
635650 HisTALON™ Superflow Cartridges 5 x 1 mL Cartridges $158.00 License Statements
635682 HisTALON™ Superflow Cartridges 5 x 5 mL Cartridges $567.00 License Statements
 

 

 


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