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Protein Expression & Purification


Products >  Protein_Expression_and_Purification >  His-Tagged_Protein_Purification >  Cobalt_Column-Bacterial_Culture

His-Tagged Protein Purification from Bacterial Cultures—TALON Single Step

TALON Single Step Columns simplify purification of his-tagged proteins. These columns combine our exclusive TALON xTractor Buffer with the patented TALON resin, to consolidate the preliminary purification steps: cell lysis, centrifugation, and resin binding. These columns provide a convenient method of purifying material for preliminary characterization of your protein of interest.

Easy, Fast, and Pure

The entire process, from culture to purified protein, can be completed in less than 1 hour.

  1. Add the culture containing your his-tagged protein to the column and then mix for 20 minutes. The column contains a dried version of TALON xTractor Buffer, which lyses cells and releases your his-tagged target protein. Our proprietary TALON Resin binds his-tags with higher specificity than any other IMAC resin, so you are assured of obtaining a high degree of purification.
  2. After the unbound cellular debris is washed from the column, your highly pure his-tagged fusion protein is eluted using standard conditions.

Looking to purify smaller amounts of protein? The 5-ml columns routinely provide up to 0.5 mg of purified protein and the 20-ml columns regularly provide up to 3.0 mg of purified protein.

Value and Versatility

These time-saving columns are more convenient than the typical method of extracting and purifying his-tagged protein over a standard TALON Resin Column. Any 6xHis-, 6xHN-, or HAT-tagged protein can be purified using either a gravity flow or a spin column method. It is easy to run several columns in parallel at room temperature, to isolate various proteins at the same time.

  At-A-Glance   Documents   Images & Data   Resources

Features

Simple purification of his-tagged proteins

  • Complete extraction and purification in under 1 hour
  • Purify target protein directly from bacterial culture
  • Ready-to-use, prepacked columns
  • Two sizes of columns available, for large and small scale purification

Applications

  • Preliminary protein characterization
  • Fast capture of his-tagged proteins

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
635632 TALON® Single Step Columns (20 ml) 10 Columns $293.00 License Statements
635628 TALON® Single Step Columns (5 ml) 25 Columns $278.00 License Statements
635631 TALON® Single Step Columns (5 ml) 2 Columns $49.00 License Statements
 

 

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