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Protein Expression & Purification


Products >  Protein_Expression_and_Purification >  Baculovirus_Expression >  Insect_Cells_and_Insect_Media

Insect Cell Culture for Baculovirus Expression

Clontech offers a cell line for insect cell culture and media formulated for the special requirements of Baculovirus Expression Systems: IPLB-Sf21 Cells and BacPAK Grace’s Basic Medium.

IPLB-Sf21 Insect Cells

These cells are used as a host for propagating the Autographa californica multiple-enveloped nuclear polyhedrosis virus (AcMNPV) and its expression vector derivatives generated from our BacPAK system. The IPLB-Sf21 cell line is derived from pupal ovaries of the fall armyworm, Spodoptera frugiperda.

BacPAK Grace’s Basic Medium

This is a base medium without any proteinaceous supplements. Because the presence of protein can inhibit transfection, use of this medium helps to maximize transfection efficiency. BacPAK Grace’s Basic Medium can easily be supplemented to make TNM-FH medium.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • Compatible with the BacPAK Baculovirus Expression System and other baculoviral systems

Applications

  • Growing Spodoptera frugiperda insect cells (Sf21 or Sf9)
  • Transfecting insect cells

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price Units Select
631404 BacPAK™ Grace's Basic Medium 500 mL $131.00
631411 IPLB-Sf21 Insect Cells 1 Vial $96.00
 

 

 
 

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