Clontech offers an insect cell line and media formulated for the special requirements of Baculovirus Expression Systems: IPLB-Sf21 Cells and BacPAK Grace’s Basic Medium.
IPLB-Sf21 Insect Cells
These cells are used as a host for propagating the Autographa californica multiple-enveloped nuclear polyhedrosis virus (AcMNPV) and its expression vector derivatives generated from our BacPAK system. The IPLB-Sf21 cell line is derived from pupal ovaries of the fall armyworm, Spodoptera frugiperda.
BacPAK Grace’s Basic Medium
This is a base medium without any proteinaceous supplements. Because the presence of protein can inhibit transfection, use of this medium helps to maximize transfection efficiency. BacPAK Grace’s Basic Medium can easily be supplemented to make TNM-FH medium.