Takara's Human Papillomavirus PCR Typing Set is designed for the identification of a broad range of human papillomavirus (HPV) types using HPV type-specific PCR. The Human Papillomavirus PCR Typing Set uses two pairs of consensus primers that are designed to amplify the E6 and E7 homologous regions of HPV (228-268 bp). Malignant HPV-16, -18, -33, -52b and -58 types are amplified using the HPVpU-1M/HPVpU-2R primer pair; benign HPV-6 and -11 types are amplified using the HPVpU-31B/HPVpU-2R primer pair. Digestion of PCR products by restriction enzymes Acc I, Afa I, Ava I, Ava II and Bgl II, available as Enzyme Set A, followed by agarose gel electrophoresis identifies the resident HPV types. The Human Papillomavirus PCR Typing Set is optimized for use with TaKaRa Taq DNA Polymerase, which can be purchased separately.