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Products >  PCR >  Long_PCR >  One-Shot_LA_PCR_Mix

One-Shot LA PCR Mix

The One-Shot LA PCR Mix enables long range PCR. This long and accurate PCR enzyme premix is composed of Takara LA Taq DNA Polymerase, LA PCR Buffer II, and dNTPs provided at 2X concentration in 25 µL aliquots. These aliquots are pre-dispensed into 0.2 mL PCR tubes. Simply add an equal volume of solution containing DNA template and primers to each 0.2 mL PCR tube to obtain a complete and ready-to-amplify reaction.

  At-A-Glance   Documents

Features

  • Convenient and reliable long range PCR
  • Longer and more accurate genomic PCR amplifications
  • Increased reproducibility and reduced optimization for long PCR

Components

RR004

One-Shot LA PCR Mix* 24 x 25 µL

*Contains Takara LA Taq, LA PCR Buffer II (2K conc; including 5 mM Mg2+) and dNTPs.

Storage

–20°C. Avoid repeated freeze-thaws by aliquoting into separate tubes and store at –20°C.

PCR Products

PCR products generated with Takara LA Taq contain a mixture of 3'-A overhangs and blunt ends, allowing >80% cloning efficiency into T-vectors. However, the cloning efficiency of longer inserts (>5 kb) into T-vectors is quite low.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
RR004 One Shot LA PCR™ Mix Ver. 2.0 1 Set $124.00 License Statements
 

 

 
 

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