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Products >  PCR >  High_Yield_PCR >  Advantage_2_Polymerase

High Fidelity PCR | Library Construction & RACE PCR | Advantage 2 Proofreading Polymerase Mixes & PCR Kits

Advantage 2 Polymerase Mix is an optimized blend of PCR enzymes that includes a proofreading DNA polymerase and our highly robust, hot start enzyme Titanium Taq DNA Polymerase. The combination provides a proven balance of high yield and high fidelity for cDNA amplification and library construction that requires minimal optimization. Advantage 2 is an extremely versatile polymerase mix that can readily amplify a wide range of DNA templates, including long PCR templates up to 18 kb. With three times higher fidelity than regular Taq, Advantage 2 polymerase mix is also perfect for PCR applications requiring accuracy, such as RACE PCR, cDNA amplification, library construction, and PCR Subtraction. Advantage 2 Polymerase Mix is highly recommended for applications downstream of SMART(er) cDNA synthesis.

The Advantage 2 Polymerase Mix consists of:

  • Titanium Taq DNA Polymerase—a robust, sensitive enzyme that provides higher yields than Taq, and contains TaqStart Antibody for convenient hot start PCR.
  • Proofreading polymerase—for longer PCR products and increased fidelity.

The Advantage 2 Polymerase Mix is available by itself (with an optimized buffer), in a complete PCR kit that includes dNTPs and PCR controls, or in a ready-to-use, lyophilized format (High Fidelity PCR EcoDry Premix).

  At-A-Glance   Documents   Images & Data   Resources


  • Unique blend of enzymes provides nearly error-free amplification—almost 3-fold higher fidelity than wild-type Taq
  • Exceptionally high yields and sensitivity with almost any target DNA up to 5 kb
  • Pre-optimized buffer eliminates the need to optimize reaction conditions
  • Integrated hot start Ab allows room temperature reaction set-up and increased specificity
  • Available in a lyophilized format as High Fidelity PCR EcoDry Premix, which is ideal for room temperature storage and convenience


  • Long and accurate PCR
  • Preparative PCR
  • cDNA synthesis/amplification
  • cDNA subtraction
  • Hot start PCR

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Cat. # Product Contents Size Price Units Select
639137 10X Advantage® 2 PCR Buffer 2 x 600 uL $73.00
639138 10X Advantage® 2 PCR Buffer 10 mL $163.00
639148 10X Advantage® 2 SA PCR Buffer 10 mL $151.00
639206 Advantage® 2 PCR Kit 100 Rxns $432.00
639207 Advantage® 2 PCR Kit 30 Rxns $138.00
639201 Advantage® 2 Polymerase Mix 100 Rxns $249.00
639202 Advantage® 2 Polymerase Mix 5 x 100 Rxns $1,000.00
639282 High Fidelity PCR EcoDry™ Premix 24 Rxns $59.00
639280 High Fidelity PCR EcoDry™ Premix 48 Rxns $97.00



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