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Products >  PCR >  General_Purpose >  Advantage_Polymerase

Advantage PCR Kits

Advantage PCR Kits contain Advantage Polymerase Mix—an enzyme blend consisting of an N-terminal deletion mutant of Taq DNA Polymerase (Barnes 1992, Barnes 1994), a proofreading enzyme, and TaqStart Antibody for automatic hot start PCR. Benefits over native Taq DNA Polymerase include greater efficiency and sensitivity, amplification of longer products, built-in hot start, and increased fidelity. The kits also come with an optimized buffer, dNTP mix, and control primers and template.

Our Advantage products have been optimized to produce high yields and long reads. Compared to regular Taq polymerase, Advantage Polymerase Mix provides more efficient amplification over a wide range of template concentrations. In addition, the optimal range of Mg2+ concentration is broader for Advantage Polymerase Mix than it is for most other enzymes, eliminating the need to spend time optimizing reaction conditions.

This enzyme mix has been further optimized for complex templates in our Advantage GC cDNA Polymerase Mixes and Kits and for high fidelity PCR in our Advantage HF PCR Kits.

  At-A-Glance   Documents   Images & Data


  • Exhibit high efficiency and sensitivity
  • Amplify up to 5 kb from genomic DNA and up to 10 kb from cDNA
  • Built-in hot start for higher specificity and lower background
  • Optimized for applications involving cDNA


  • Long and accurate PCR
  • cDNA PCR
  • RACE
  • Preparative PCR
  • Hot-start PCR
  • GC-rich PCR (GC)
  • High-fidelity PCR (HF)


Barnes, W. M. The fidelity of Taq polymerase catalyzing PCR is improved by an N-terminal deletion. Gene 112, 29–35 (1992).

Barnes, W. M. PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates. Proc. Natl. Acad. Sci. U. S. A. 91, 2216–2220 (1994).

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Cat. # Product Contents Size Price Units Select
639206 Advantage® 2 PCR Kit 100 Rxns $432.00



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