Advantage PCR Kits contain Advantage Polymerase Mix—an enzyme blend consisting of an N-terminal deletion mutant of Taq DNA Polymerase (Barnes 1992, Barnes 1994), a proofreading enzyme, and TaqStart Antibody for automatic hot start PCR. Benefits over native Taq DNA Polymerase include greater efficiency and sensitivity, amplification of longer products, built-in hot start, and increased fidelity. The kits also come with an optimized buffer, dNTP mix, and control primers and template.
Our Advantage products have been optimized to produce high yields and long reads. Compared to regular Taq polymerase, Advantage Polymerase Mix provides more efficient amplification over a wide range of template concentrations. In addition, the optimal range of Mg2+ concentration is broader for Advantage Polymerase Mix than it is for most other enzymes, eliminating the need to spend time optimizing reaction conditions.
This enzyme mix has been further optimized for complex templates in our Advantage GC cDNA Polymerase Mixes and Kits and for high fidelity PCR in our Advantage HF PCR Kits.