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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  Selection_Guides >  MACHEREY_NAGEL_Selection_Guide

Nucleic Acid Purification Selection Guides

Use the list below to choose the right nucleic acid purification kit.

RNA Purification

Total RNA from Cells and Tissues

Starting material Typical yield Format Automated or manual protocol Comparable Qiagen kit You need
  • <5 x 106 cultured cells
  • <109 bacterial cells
  • <30 mg tissue
  • up to 108 yeast cells
14–70 µg Mini spin columns M 74104/74106 NucleoSpin RNA
  • 1–100,000 cultured cells
  • <5 mg tissue
0.1–1,500 ng XS spin columns M 74004 NucleoSpin RNA XS
  • <5 x 107 cultured cells
  • <1010 bacterial cells
  • 200 mg tissue
  • <3 x 108 yeast cells
  • 180 µg from 107 HeLa cells
  • 620 µg from 4 x 107 HeLa cells
Midi spin columns M 75142 NucleoSpin RNA Midi
  • <107 cultured cells
  • 30 mg tissue
<100 µg 8-well strips M NucleoSpin 8 RNA
  • <107 cultured cells
  • 30 mg tissue
<100 µg 96-well plates M/A 74182 NucleoSpin 96 RNA
  • 20 mg tissue
  • 2 x 106 cells
<30 µg Magnetic M/A 956336 NucleoMag 96 RNA

Total RNA from Viruses

Sample volume Format Automated or manual protocol Comparable Qiagen kit You need
150 µl–1 ml serum, plasma, or cell-free biological fluids Mini spin columns M 52904/52906 NucleoSpin RNA Virus
150 µl (100 cp/ml) serum, plasma, or cell-free biological fluids 8-well strips M/A NucleoSpin 8 Virus
150 µl (100 cp/ml) serum, plasma, or cell-free biological fluids) 96-well plates M/A NucleoSpin 96 Virus
200 µl serum or plasma Magnetic M/A 955235 NucleoMag 96 Virus
Starting material Format Automated or manual protocol Comparable Qiagen kit You need
  • 4–10 sections (10 µm) of up to 6 mm2 total area
  • <50 mg tissue
Mini spin columns M 73504 NucleoSpin totalRNA FFPE
  • 1–3 sections (10 µm) of up to 6 mm2 total area
  • <5 mg tissue
XS spin columns M/A NucleoSpin totalRNA FFPE XS

MicroRNA

Starting Material Format Automated or manual protocol Comparable Qiagen kit You need
  • <107 cultured cells
  • <30 mg human/animal tissue
  • <50 mg plant tissue
  • <150 µl reaction mixture
Mini spin columns M 217004 NucleoSpin miRNA
300 µl plasma or serum (up to 900 µl with multiple loadings) Mini spin columns M 217184 NucleoSpin miRNA Plasma

RNA/DNA Protein

Co-purify Format Automated or manual protocol Comparable Qiagen kit You need
Total RNA and genomic DNA Midi gravity-flow columns M 80204 NucleoBond RNA/DNA
Total RNA and protein Mini spin columns M 80404 NucleoSpin RNA/Protein

Plasmid Purification

Plasmid Minipreps

Format Typical yield Sample volume (high copy number plasmid) Automated or manual protocol Comparable Qiagen kit You need
Miniprep spin columns <40 µg 1–10 ml E. coli culture M 27104/27106 NucleoSpin Plasmid
8-well strips <20 µg 1–5 ml E. coli culture M/A NucleoSpin 8 Plasmid
96-well plates <20 µg 1–5 ml E. coli culture M/A 27191/27193 NucleoSpin 96 Plasmid
Mini anion-exchange columns* 20 µg 1–10 ml E. coli culture M 10023 NucleoBond PC 20
Ultra fast minipreps <35 µg 2–10 ml E. coli culture M NucleoSpin Plasmid EasyPure
Fast miniprep spin columns <15 µg 1–3 ml E. coli culture M NucleoSpin Plasmid QuickPure

* NucleoBond PC20 provides highly pure plasmid DNA, suitable for transfecting or sequencing.

Plasmid Midipreps

Format Typical yield Sample volume (high copy number plasmid) Automated or manual protocol Comparable Qiagen kit You need
Fast, midi anion-exchange columns with column filters and finalizers* 250 µg 5–200 ml E. coli culture M 12643 NucleoBond Xtra Midi Plus
Midi anion-exchange columns with column filters 250 µg 5–200 E. coli culture M 12243/12245 NucleoBond Xtra Midi
Midi anion-exchange columns with folded filters 100 µg 5–30 ml E. coli culture M 12143 NucleoBond PC 100

* The Finalizer eliminates centrifugation and reduces precipitation time from >1 hr to just 5 min.

Plasmid Maxipreps

Format Typical yield Sample volume (high copy number plasmid) Automated or manual protocol Comparable Qiagen kit You need
Fast, maxi anion-exchange columns with column filters and finalizers* 1,000 µg 600 ml E. coli culture M 12662/12663 NucleoBond Xtra Maxi Plus
Maxi anion-exchange columns with column filters 1,000 µg 600 ml E. coli culture M 12262/12263 NucleoBond Xtra Maxi
Maxi anion-exchange columns with folded filters 500 µg 30–150 ml E. coli culture M 12162/12163/12165 NucleoBond PC 500

* The Finalizer eliminates centrifugation and reduces precipitation time from >1 hr to just 5 min.

Endotoxin-Free Plasmid DNA

Sample volume (high copy number plasmid) Typical yield Format Automated or manual protocol Comparable Qiagen kit You need
200 ml E. coli culture >250 µg Rapid, midi anion-exchange columns with column filters and finalizers M NucleoBond Xtra Midi Plus EF
200 ml E. coli culture >250 µg Midi anion-exchange columns with column filters M NucleoBond Xtra Midi EF
600 ml E. coli culture >1,000 µg Rapid, maxi anion-exchange columns with column filters and finalizers* M NucleoBond Xtra Maxi Plus EF
600 ml E. coli culture >1,000 µg Maxi anion-exchange columns with column filters M NucleoBond Xtra Maxi EF
150 ml E. coli culture 450–520 µg Maxi anion-exchange columns with folded filters M 12362 NucleoBond PC 500 EF
2,000 ml E. coli culture 1,700–2,000 µg Mega anion-exchange columns M 12381 NucleoBond PC 2000 EF
2,000 ml E. coli culture 5,000–10,000 µg Giga anion-exchange columns M 12391 NucleoBond PC 10000 EF

* The Finalizer eliminates centrifugation and reduces precipitation time from >1 hr to just 5 min.

BAC

Sample volume Format Automated or manual protocol Comparable Qiagen kit You need
1.3–3.9 ml E. coli culture (BACs) 96-well plates M/A 26173 NucleoSpin 96 Flash
250–750 ml E. coli culture Maxi anion-exchange columns with column filters M NucleoBond Xtra BAC
100–500 ml E. coli culture Maxi anion-exchange columns with folded filters M 12462 NucleoBond BAC 100

Genomic DNA Purification

Tissue

Starting material Format Automated or manual protocol Comparable Qiagen kit You need
1–25 mg tissue Mini spin columns M/A 69504/69506 NucleoSpin Tissue*
0.025–10 mg tissue XS spin columns M 56304 NucleoSpin Tissue XS*
<20 mg tissue 8-well strips M/A NucleoSpin 8 Tissue*
<20 mg tissue 96-well plates M/A NucleoSpin 96 Tissue*
<20 mg tissue Magnetic beads M/A 95336 NucleoMag 96 Tissue*
<20 mg tissue Mini anion-exchange columns M 10223 NucleoBond AXG 20
<100 mg tissue Midi anion-exchange columns M 10243 NucleoBond AXG 100
<400 mg tissue Maxi anion-exchange columns M 10262 NucleoBond AXG 500

* Use NucleoSpin Tissue and NucleoMag Tissue to isolate DNA from tissue (e.g., mouse tails), cells (e.g., cultured human or animal cells, bacteria, yeast), forensic samples (e.g., dried blood spots, hair, buccal swabs, cigarette stubs), FFPE tissue, or blood sample storage cards (e.g., NucleoSave).

Plant

Use NucleoSpin Plant and NucleoMag Plant to isolate DNA from plant cells, plant tissues, and fungi.

Starting material Format Automated or manual protocol Comparable Qiagen kit You need
<100 mg tissue Mini spin columns M 69104/69106 NucleoSpin Plant II
<250 mg plant tissue Midi spin columns M NucleoSpin Plant II Midi
<1,000 mg plant tissue Maxi spin columns M 68163 NucleoSpin Plant II Maxi
20–100 mg plant tissue 8-well strips M/A NucleoSpin 8 Plant II
20–100 mg plant tissue 96-well plates M/A NucleoSpin 96 Plant II
20–50 mg plant tissue Magnetic beads M/A NucleoMag 96 Plant

Blood

Use these kits to isolate DNA from whole blood (treated with citrate, EDTA, heparin, or CPDA), serum, plasma, buffy coats, platelets, or body fluids (e.g., amniotic fluid).

Sample volume Format Automated or manual protocol Comparable Qiagen kit You need
1–200 µl whole blood Mini spin columns M 51104/51106 NucleoSpin Blood
0.2–2 ml whole blood Midi spin columns M 51183/51185 NucleoSpin Blood L
2–10 ml whole blood Maxi spin columns M 51192/51194 NucleoSpin Blood XL
1–200 µl whole blood Fast mini spin columns M NucleoSpin Blood QuickPure
2–5 ml whole blood Midi anion-exchange columns M 13343 NucleoBond CB 100
5–20 ml whole blood Maxi anion-exchange columns M 13362 NucleoBond CB 500
1–200 µl whole blood 8-well strips M/A NucleoSpin 8 Blood
1–200 µl whole blood 96-well plates M/A 51161 NucleoSpin 96 Blood
200 µl whole blood Fast 8-well strips M NucleoSpin 8 Blood QuickPure
200 µl whole blood Fast 96-well plates M 51161 NucleoSpin 96 Blood QuickPure
200 µl whole blood Magnetic beads M/A 951436 NucleoMag Blood

Plasma

Use these kits to isolate DNA from whole blood (treated with citrate, EDTA, heparin, or CPDA), serum, plasma, buffy coats, platelets, or body fluids (e.g., amniotic fluid).

Forensic Samples

Purify genomic DNA from forensic samples, buccal swabs, and blood spots.

Format Elution volume Automated or manual protocol Comparable Qiagen kit You need
Funnel columns 100 µl M NucleoSpin DNA Trace
8-well strips 50–100 µl M/A NucleoSpin 8 Trace
96-well plates 50–100 µl M/A NucleoSpin 96 Trace
Magnetic beads >50 µl M/A NucleoMag 96 Trace

Food

Format Starting material Automated or manual protocol Comparable Qiagen kit You need
Mini spin columns Up to 200 mg food or feed M NucleoSpin Food
8-well strips Up to 200 mg food or feed M/A NucleoSpin 8 Food
96-well plates Up to 200 mg food or feed M/A NucleoSpin 96 Food

DNA Clean-Up

Gel Extraction

Format Downstream applications Automated or manual protocol Comparable Qiagen kit You need
Mini spin columns Sequencing, cloning M 28704 NucleoSpin Gel and PCR Clean-Up
Silica bead suspension Sequencing, cloning M 20021 NucleoTrap

PCR Clean-Up

Format Downstream applications Automated or manual protocol Comparable Qiagen kit You need
Mini spin columns Sequencing, cloning M 28104 NucleoSpin Gel and PCR Clean-Up
8-well strips Sequencing, cloning M NucleoSpin 8 PCR Clean-Up
96-well plates Sequencing, cloining M/A 28181 NucleoSpin 96 PCR Clean-Up
Magnetic bead suspension Sequencing, small elution volumes M/A NucleoMag 96 PCR
96-well plates Sequencing, microarray spotting M/A 28051 NucleoFast 96 PCR Plates
Silica bead suspension Sequencing, cloning M 20902 NucleoTraPCR
Suspension Resin-based enzyme protocol M QuickClean

High-Throughput DNA and RNA Purification

DNA Clean-Up

Starting material Format Automated or manual protocol Comparable Qiagen kit You need
PCR reaction 96-well plates M/A 28181 NucleoSpin 96 PCR Clean-Up
PCR reaction Magnetic bead suspension M/A NucleoMag 96 PCR
PCR reaction Fast 96-well plates M/A 28181 NucleoFast 96 PCR Plates

Genomic DNA

Starting material Format Automated or manual protocol Comparable Qiagen kit You need
Cells and tissues 96-well plates M/A NucleoSpin 96 Tissue
Cells and tissues Magnetic bead suspension M/A NucleoMag 96 Tissue
Plants 96-well plates M/A NucleoSpin 96 Plant II
Plants Magnetic beads M/A NucleoMag 96 Plant
Blood 96-well plates M/A 51161 NucleoSpin 96 Blood
Blood Magnetic Beads M/A NucleoMag Blood
Forensic samples, buccal swabs, blood spots, cigarette filters 96-well plates M/A NucleoSpin 96 Trace
Forensic samples, buccal swabs, blood spots, cigarette filters Magnetic beads M/A NucleoMag 96 Trace
Food 96-well plates M/A NucleoSpin 96 Food

Plasmid

Type of DNA Format Automated or manual protocol Comparable Qiagen kit You need
Plasmid DNA 96-well plates M/A 27191/27193 NucleoSpin 96 Plasmid
BAC DNA 96-well plates M/A 26173 NucleoSpin 96 Flash

RNA

Souce of RNA Format Automated or manual protocol Comparable Qiagen kit You need
Cells and tissues 96-well plates M/A 74182 NucleoSpin 96 RNA
Cells and tissues Mini spin columns (for the QIAcube) M/A 74116 NucleoSpin RNA II
Cells and tissues Magnetic beads M/A 956336 NucleoMag 96 RNA
Viruses 96-well plates M/A NucleoSpin 96 Virus
Viruses Magnetic beads M/A 955235 NucleoMag 96 Virus

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