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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  High-Throughput >  Plasmid >  Miniprep

Multi-Prep Plasmid DNA Purification—NucleoSpin 8/96 Plasmid & Core Kits

The NucleoSpin 96 Plasmid Kits are designed for purifying up to 20 µg of high-copy plasmids from 1–5 ml E. coli overnight culture, preferably grown in LB medium. The NucleoSpin Plasmid Binding Strips/Plates contain a unique silica membrane that binds DNA in the presence of chaotropic salt. 96-well plates can be processed manually, under vacuum, or by centrifugation.

The NucleoSpin 8/96 Plasmid procedure is a modified version of the Birnboim and Doly alkaline lysis plasmid miniprep protocol. Bacterial cultures are harvested by an initial centrifugation step. After resuspension of the pelleted bacteria (Buffer A1) and alkaline cell lysis (Buffer A2), a neutralization and binding buffer (Buffer A3) containing chaotropic salts is added. The resulting bacterial crude lysates are cleared by vacuum filtration with the NucleoSpin Plasmid Filter Strips/Plate. The cleared lysates containing the plasmid DNA are collected in the NucleoSpin Plasmid Binding Strips/Plate. The chaotropic salt enables a reversible adsorption of plasmid DNA to the NucleoSpin silica membrane during the second vacuum filtration step. High purity is achieved in the final plasmid DNA preparation by complete removal of cellular contaminants, salts, detergents, and other compounds in subsequent washing steps. Highly pure plasmid DNA is eluted with Elution Buffer AE (5 mM Tris / HCl, pH 8.5) or water (pH 8.0– 8.5), and can directly be used for downstream applications.

At-A-Glance Documents Images & Data Resources

Features

Plasmid DNA purification in a flexible 8-well strip format, or a high-throughput 96-well plate format.

  • Time-saving parallel isolation of plasmid DNA
  • Processing of up to 5 ml bacterial culture
  • NucleoSpin 8 Filter Strips/96 Filter Plates provide convenient filtration of bacterial lysates
  • Suitable for manual or automated processing under vacuum, or by centrifugation
  • Innovative MN Wash Plate minimizes risk of cross-contamination
  • NucleoSpin 8/96 Plasmid Core Kits supply basic content focused on automation platforms*
    * Additional accessories can be combined as needed

Applications

  • Manual and automated isolation of high-copy plasmids
  • Support protocol available for purification of cosmids
  • Typical downstream applications: cloning, sequencing, PCR, transformation, restriction analysis

Product Overview

Products NucleoSpin 8 Plasmid
NucleoSpin 8 Plasmid Core Kit
NucleoSpin 96 Plasmid
NucleoSpin 96 Plasmid Core Kit
Technology Silica-membraneSilica-membrane
Format 8-well strips96-well plates
Processing Manual, automated,
vacuum
Manual, automated,
vacuum, centrifugation
Lysate clarification 8-well filter strips96-well filter plates
Sample material 1–5 ml E. coli culture1–5 ml E. coli culture
Vector size <15 kbp<15 kbp
Typical yield 4–6 µg/ml E. coli culture4–6 µg/ml E. coli culture
A260/A280 1.70–1.851.70–1.85
Elution volume 75–150 µl75–150 µl
Preparation time 45 min/6 strips45 min/plate
Binding capacity 20 µg20 µg

Vacuum Processing

NucleoSpin 8/96 Plasmid kits can be used manually with the NucleoVac 96 Vacuum Manifold. The manual procedure requires a suitable centrifuge (for plates or tubes) in order to harvest the bacteria.
Starter Set A (containing Column Holders A and NucleoSpin Dummy Strips) is required for processing 8-well strips and 96-well plates under vacuum.

NucleoSpin 8 Plasmid Kit Formats

NucleoSpin 8 PlasmidNucleoSpin 8 Plasmid Core Kit
NucleoSpin Plasmid Filter Strips NucleoSpin Plasmid Filter Strips
BuffersBuffers
RNase ARNase A
MN Wash Plate __
Culture Plate__
Rack of Tube Strips__

NucleoSpin 96 Plasmid Kit Formats

NucleoSpin 96 PlasmidNucleoSpin 96 Plasmid Core Kit
NucleoSpin Plasmid Filter Strips NucleoSpin Plasmid Filter Strips
BuffersBuffers
RNase ARNase A
MN Wash Plate __
MN Square-well Block__
Elution Plate U-bottom__
Gas-permeable Foil __
Self-adhering Foil__

 
 
Products
Cat. # Product Package Size Price # of Units Select
740621 NucleoSpin® 8 Plasmid 12 x 8 Preps $242.00
740621.5 NucleoSpin® 8 Plasmid 60 x 8 Preps $1,164.00
740625.24 NucleoSpin® 96 Plasmid 24 x 96 Preps $4,202.00
740625.4 NucleoSpin® 96 Plasmid 4 x 96 Preps $840.00
740625.1 NucleoSpin® 96 Plasmid 1 x 96 Preps $231.00
740461.4 NucleoSpin® 8 Plasmid Core Kit 48 x 8 Preps $667.00
740503.1 NucleoPrep Anion II resin 1 kg $3,536.00
740616.4 NucleoSpin® 96 Plasmid Core Kit 4 x 96 Preps $735.00
 

 

 

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