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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  Genomic_DNA >  Stool

Isolation of genomic DNA from stool samples—NucleoSpin DNA Stool

NucleoSpin DNA Stool is designed for purification of genomic DNA from bacterial and epithelial cells present in stool samples. The isolated DNA is used for analysis of the microbiome or genotyping of the donor. The kit utilizes NucleoSpin Bead Tubes Type A (ceramic beads; included in the kit) in combination with a common disruption device, e.g., an MN Bead Tube Holder or a swing mill, to lyse different cell types and tissues using a single procedure. NucleoSpin Inhibitor Removal Columns are included for convenient elimination of contaminants.

  At-A-Glance   Documents

Features

  • Suitable for any stool sample—high-quality DNA from stool from carnivores, omnivores, and herbivores
  • NucleoSpin Bead Tubes for efficient lysis included—compatible with the most common disruption devices
  • NucleoSpin Inhibitor Removal Columns ensure superior DNA purity

Applications

  • Purification of genomic DNA from bacterial and epithelial cells present in stool samples
  • Typical downstream applications: PCR, real-time PCR, Southern blotting, microarray technology

Product Overview

Technology Silica membrane technology combined
with NucleoSpin Bead Tubes Type A
Format Mini spin columns
Starting material Approximately 200 mg (180–220 mg)*
stool samples (fresh or frozen)
Fragment size 200 bp–approximately 50 kb
Typical yield Depends on sample type, quality, and water content
A260/A280 1.7–1.9
Elution volume 30–100 µl
Preparation time 60 min/10 preps
Binding capacity 50 µg

*For human stool samples, approximately 200 mg should be used. For animal stool samples, depending on the species, a smaller amount of sample material may be required for optimal results.


 
 
Products
New Cat. # Product Contents Size Price Units Select
740472.50 NucleoSpin® DNA Stool 50 Preps $252.00
 

 

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