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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  Genomic_DNA >  RapidLyse >  HTP

Rapid, high-throughput extraction of genomic DNA—NucleoSpin 96 DNA RapidLyse

The isolation of genomic DNA (gDNA) from most organs is a challenging task. Organs are composed of different tissues with various types of cells and matrices. Large molecules such as collagens and proteoglycans can impede lysis and inhibit downstream enzymatic reactions such as PCR. The new NucleoSpin 96 DNA RapidLyse kit uses a unique lysis chemistry to release gDNA from organs and cells with the highest efficiency. This kit provides high-throughput DNA isolation for all common liquid handling platforms.

  At-A-Glance   Documents

Features

  • Unique lysis chemistry efficiently releases gDNA from cells, tissues, and organs
  • Manual or automated processing by vacuum, positive pressure, or centrifugation  
  • Easy automation on robotic platforms
  • Also available in a single prep spin column format

Applications

  • Manual or automated isolation of DNA from human and animal tissue, cultured cells, or bacteria

Product Overview

Technology Silica membrane technology
Format96-well plates
ProcessingCentrifuge, positive pressure, or vacuum (manual or automated)
Starting material Fresh, frozen, dried, or ethanol-preserved organs
Eukaryotic cells
Tail or ear clippings
Sample amount Up to 30 mg fresh weight, 1 x 106 cells
Lysis time 60 min or less
Preparation time 60 min/96 preps (excluding lysis)
Typical yield Up to 4 μg of DNA per mg tissue
Elution volume 100 µl
A260/280 1.7–1.9
Binding capacity 40 µg

 
 
Products
New Cat. # Product Contents Size Price Units Select
740110.1 NucleoSpin® 96 DNA RapidLyse 96 Preps $261.00
740110.4 NucleoSpin® 96 DNA RapidLyse 4 Plates $862.00
 

 

 
 

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