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Molecular Biology Tools

Products >  Molecular_Biology_Tools >  Restriction_Enzymes >  Restriction_Enzymes_wz >  XhoI


DNA restriction enzymes from Takara Bio are unsurpassed in quality and purity. We’ve been producing them for over 30 years and were the first manufacturers to offer commercially available restriction enzymes in Japan. Each lot of every DNA restriction enzyme undergoes stringent quality control tests. Takara Bio DNA restriction enzymes are supplied with optimized buffers that provide maximum activity during restriction enzyme digestion. Visit our Restriction Enzyme Applications pages for information on how to digest DNA, relative activity in each Universal Buffer, star activity, buffers for double digestion, effects of DNA methylation, how to inactivate enzymes, and more.

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DNA Restriction Enzyme: XhoI

  • Source: Xanthomonas holcicola
  • Concentration: 4-12 units/µ [High concentration: 30-60 units/µl]
  • Supplied buffer: H
  • Reaction temperature: 37°C
  • Substrate for unit definition: lambda DNA
  • Genome DNA analysis: Escherichia coli genome DNA
  • Ligation-recutting test: The ligation efficiency of cohesive end DNA fragments generated by this enzyme will be low. More efficient ligation can be achieved using reaction conditions for blunt end ligation.
  • Effect of DNA methylation: Enzyme activity is affected by CG methylase. (It cleaves CT5m CGAG very slowly.)

TaKaRa follows a stringent QC process when manufacturing their restriction enzymes. Each lot of every enzyme undergoes four quality tests including: overdigestion, Genome DNA analysis, Ligation-Recutting and pKF3 Cloning.

Cat. # Product Contents Size Price Units Select
1094AH Xho I (High Concentration) 5,000 Units $69.00
1094A Xho I 5,000 Units $69.00
1094B Xho I 25,000 Units $275.00
1094BH Xho I (High Concentration) 25,000 Units $275.00


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