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Sau3AI (MboI)

DNA restriction enzymes from Takara Bio are unsurpassed in quality and purity. We’ve been producing them for over 30 years and were the first manufacturers to offer commercially available restriction enzymes in Japan. Each lot of every DNA restriction enzyme undergoes stringent quality control tests. Takara Bio DNA restriction enzymes are supplied with optimized buffers that provide maximum activity during restriction enzyme digestion. Visit our Restriction Enzyme Applications pages for information on how to digest DNA, relative activity in each Universal Buffer, star activity, buffers for double digestion, effects of DNA methylation, how to inactivate enzymes, and more.

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DNA Restriction Enzyme: Sau3AI (MboI)

  • Source: Staphylococcus aureus 3A
  • Concentration: 4-12 units/µl [High concentration: 30-60 units/µl]
  • Supplied buffer: H
  • Reaction temperature: 37°C
  • Substrate for unit definition: lambda DNA
  • Effect of DNA methylation: Enzyme activity is affected by CG methylase depending on the sequence following the recognition site, but is not affected by dam methylase.
  • Star Activity: Cleavage at alternative site(s) occurs the presence of DMSO or high concentrations of glycerol. Known alternative recognition sites include: SATC, GMTC, GAKC, and GATS (where S represents G or C; M represents A or C; and K represents G or T). 

TaKaRa follows a stringent QC process when manufacturing their restriction enzymes. Each lot of every enzyme undergoes four quality tests including: overdigestion, Genome DNA analysis, Ligation-Recutting and pKF3 Cloning

Cat. # Product Contents Size Price Units Select
1082A Sau3A I (Mbo I) 200 Units $52.00
1082BH Sau3A I (Mbo I) (High Concentration) 1,000 Units $213.00
1082B Sau3A I (Mbo I) 1,000 Units $213.00


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